CD73分子(CD73)活性蛋白
Active Cluster Of Differentiation 73 (CD73)
NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto
- 編號(hào)APB250Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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活性實(shí)驗(yàn)
![](http://static.cloud-clone.cn/product/pictures/APB250Hu01-201706081051253504.jpg)
5'-Nucleotidase, Ecto (NT5E), also known as ecto-5'-nucleotidase or CD73, is an enzyme catalyzing thehydrolysis of nucleoside-5’-monophosphates to nucleosides and inorganic phosphate. The enzyme is a dimer composed of 2 identical 70kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. NT5E is a marker of lymphocyte differentiation that has functions independent of its catalytic activity, such as T-cell activation and cell-cell adhesion. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from NT5E by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The enzyme is widely distributed in human and animal tissues. Besides, AF4/FMR2 Family, Member 1 (AFF1) has been identified as an interactor of NT5E thus a binding ELISA assay was conducted to detect the interaction of recombinant human NT5E and recombinant human AFF1. Briefly, NT5E were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to AFF1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NT5E pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of NT5E and AFF1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
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相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APB250Hu01 | CD73分子(CD73)活性蛋白 | Cell?culture;?Activity?Assays. |
RPB250Hu01 | CD73分子(CD73)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB250Hu01 | CD73分子(CD73)多克隆抗體 | WB; IHC; ICC; IP. |
FAB250Hu01 | 抗CD73分子(CD73)多克隆抗體 | Flow cytometry. |
LAB250Hu81 | CD73分子(CD73)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAB250Hu71 | CD73分子(CD73)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
FAB250Hu41 | 抗CD73分子(CD73)多克隆抗體(藻紅素標(biāo)記) | Flow cytometry. |
FAB250Hu51 | 抗CD73分子(CD73)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | Flow cytometry. |
FAB250Hu81 | 抗CD73分子(CD73)多克隆抗體(異硫氰酸熒光素標(biāo)記) | Flow cytometry. |
RAB250Hu21 | CD73分子(CD73)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
MAB250Hu21 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
MAB250Hu24 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
MAB250Hu25 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
MAB250Hu22 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
MAB250Hu27 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
MAB250Hu29 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
MAB250Hu23 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
SEB250Hu | CD73分子(CD73)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB250Hu | CD73分子(CD73)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB250Hu01 | CD73分子(CD73)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Journal of Autoimmunity | The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation [Pubmed:25640206] |
Talanta | Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment?… [10.1016:j.talanta.2018.01.044] |
Cancer?Biomarkers | Expression and clinical significance of serum NT5E protein in patients with colorectal cancer [Pubmed: 30932882] |