細胞色素P450家族成員1B1(CYP1B1)活性蛋白
Active Cytochrome P450 1B1 (CYP1B1)
CP1B; GLC3A; Cytochrome P450,Family 1,Subfamily B,Polypeptide 1; Glaucoma 3,Primary Infantile
- 編號APD297Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點7.9
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Cytochrome P450 family 1 subfamily B member 1?(CYP1B1) is an?enzyme, Which belongs to the?cytochrome P450?superfamily. CYP1B1 can catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. Besides, Heat Shock Protein family B (small) member 2 (HSPb2) has been identified as an interactor of CYP1B1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1B1 and recombinant human HSPb2, CYP1B1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPb2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CYP1B1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1B1 and HSPb2 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of CYP1B1 with HSPb2.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPD297Hu01 | 細胞色素P450家族成員1B1(CYP1B1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD297Hu01 | 細胞色素P450家族成員1B1(CYP1B1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAD297Hu01 | 細胞色素P450家族成員1B1(CYP1B1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAD297Hu71 | 細胞色素P450家族成員1B1(CYP1B1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAD297Hu21 | 細胞色素P450家族成員1B1(CYP1B1)單克隆抗體 | WB; IHC; ICC; IP. |
| SED297Hu | 細胞色素P450家族成員1B1(CYP1B1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD297Hu | 細胞色素P450家族成員1B1(CYP1B1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Int J Mol Sci | Lipidomic Profile and Enzymes Activity in Hepatic Microsomes of Rats in Physiological and Pathological Conditions [Pubmed:35008866] |
| ResearchSquare | Effects of herbicide atrazine on reproductive hormonal levels, Cytochrome P450, and gonadal structure of adult male and female crayfish, Procambarus clarkii [] |
