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端粒酶逆轉(zhuǎn)錄酶(TERT)活性蛋白

Active Telomerase Reverse Transcriptase (TERT)

EST2; TCS1; TP2; TRT; hEST2; Telomerase catalytic subunit; Telomerase-associated protein 2

  • 端粒酶逆轉(zhuǎn)錄酶(TERT)活性蛋白 產(chǎn)品包裝(模擬)
  • 端粒酶逆轉(zhuǎn)錄酶(TERT)活性蛋白 產(chǎn)品包裝(模擬)
  • APC241Hu01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Telomerase?reverse transcriptase?(TERT) is a subunit of the?enzyme?complex?telomerase, which adds nucleotides to the ends of?telomeres?as they become shortened during cell division?.Telomerase complex plays a key role in cancer formation by telomere dependent or independent mechanisms. According to statistics, human?telomerase reverse transcriptase?(h-TERT) is overexpressed in more than 85% of tumors with diverse histologies, with little expression in normal tissues. Expression of h-TERT correlates with activity of telomerase, which is required for the capacity for limitless replication, a hallmark of cancer. The X-Ray Repair Cross Complementing 6 (XRCC6) is high affinity receptor for TERT, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TERT and recombinant rat XRCC6. Briefly, TERT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to XRCC6-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TERT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant TERT and recombinant rat XRCC6 was shown in Figure 1, the EC50?for this effect is 0.009 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
APC241Hu01 端粒酶逆轉(zhuǎn)錄酶(TERT)活性蛋白 Cell?culture;?Activity?Assays.
RPC241Hu01 端粒酶逆轉(zhuǎn)錄酶(TERT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC241Hu02 端粒酶逆轉(zhuǎn)錄酶(TERT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC241Hu01 端粒酶逆轉(zhuǎn)錄酶(TERT)多克隆抗體 WB; IHC; ICC; IP.
MAC241Hu22 端粒酶逆轉(zhuǎn)錄酶(TERT)單克隆抗體 WB; IHC; ICC; IP.
SEC241Hu 端粒酶逆轉(zhuǎn)錄酶(TERT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC241Hu 端粒酶逆轉(zhuǎn)錄酶(TERT)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Physiological Anthropology Telomeres are elongated in rats exposed to moderate altitude [Pubmed:Pmc4088304]
Exp Ther Med Thymocytes maintain immune activity through telomere elongation in rats under hypoxic conditions [PubMed: 26640565]
Journal of Physiological Anthropology Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia [Pubmed:29454386]
Respiratory Medicine Coexistence of obstructive sleep apnea and telomerase activity, concentration of selected adipose tissue hormones and vascular endothelial function in patients with?… []
Stress Maternal neglect results in reduced telomerase activity and increased oxidative load in rats [Pubmed: 32588701]
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Reversal of brain aging by targeting telomerase: A nutraceutical approach [34515324]
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