半胱氨酸蛋白酶抑制劑B(CSTB)重組蛋白
Recombinant Cystatin B (CSTB)
Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor
- 編號(hào)RPJ322Bo01
- 物種Bos taurus; Bovine (Cattle,牛) 相同的名稱,不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞質(zhì)
- 預(yù)測(cè)分子量12.4kDa
- 實(shí)際分子量15kDa(差異分析請(qǐng)參閱說(shuō)明書)
- 片段與標(biāo)簽Met1~Phe98 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.8
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請(qǐng)參見活性蛋白。 - 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 912 ¥ 2280 ¥ 4560 ¥ 13680 ¥ 34200
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序列
![](http://static.cloud-clone.cn/product/pictures/RPJ322Bo01-201708171248165765.jpg)
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
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相關(guān)產(chǎn)品
編號(hào) | 適用物種:Bos taurus; Bovine (Cattle,牛) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPJ322Bo01 | 半胱氨酸蛋白酶抑制劑B(CSTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAJ322Bo01 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體 | WB; IHC; ICC; IP. |
LAJ322Bo81 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAJ322Bo71 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
AIDS. | Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders [PubMed: 23291538] |
International Journal of Circumpolar Health | Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer [PubMed: PMC3754495] |
PLoS One. | Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis [PubMed: PMC3365072] |
Journal of Neuroimmune Pharmacology | Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages [Pubmed:25209871] |
European Journal of Cancer Supplements | A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system [Science: Article] |
Annals of Clinical Biochemistry | Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. [pubmed:28766361] |
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