血管非炎性蛋白1(VNN1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Vanin 1 (VNN1)
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
- 編號KSC598Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, urine, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.059ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7560 ¥ 12600 ¥ 22680 ¥ 44100 ¥ 75600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對血管非炎性蛋白1(VNN1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC598Hu02 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu03 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu01 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu05 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC598Hu04 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC598Hu02 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC598Hu04 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC598Hu03 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB |
| PAC598Hu01 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB |
| LAC598Hu71 | 血管非炎性蛋白1(VNN1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAC598Hu24 | 血管非炎性蛋白1(VNN1)單克隆抗體 | WB |
| MAC598Hu21 | 血管非炎性蛋白1(VNN1)單克隆抗體 | WB |
| MAC598Hu22 | 血管非炎性蛋白1(VNN1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC598Hu | 血管非炎性蛋白1(VNN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC598Hu | 血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC598Hu01 | 血管非炎性蛋白1(VNN1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Toxicology | Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury [ScienceDirect: S0300483X11003234] |
| Journal of Pharmacology and Experimental Therapeutics | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury [Aspet: 192807] |
| J Pharmacol Exp Ther | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury. [PubMed: 22399813] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis [PubMed: 23307618] |
| Iranian Journal of Health and Physical Activity | Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in Rats [Jm.um: Source] |
| Platelets | Value of vanin-1 assessment in adult patients with primary immune thrombocytopenia [Pubmed: 23534352] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis. [Pubmed: 23307618] |
| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats [Sagepub:Source] |
| Food Chem Toxicol. | The effect of activated charcoal on adenine-induced chronic renal failure in rats [Pubmed:24412558] |
| Hypertension Research | Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intake [PubMed: 26376947] |
| Inflammation | Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell Balance [PubMed: 26253296] |
| Toxicology | Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinoma [Pubmed:27317936] |
| J Biomed Mater Res A | Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice [Pubmed:26850371] |
| APMIS | Citrate attenuates vascular calcification in chronic renal failure rats. [pubmed:28332248] |
| Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. [pubmed:29027259] |
| Cellular?Physiology and Biochemistry | Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats?… [Pubmed:29550811] |
| International Journal of Molecular?Sciences | Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis [Pubmed: 30332759] |
| International Journal of Molecular Sciences | Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats [Pubmed: 31514290] |
| The Journal of Clinical Hypertension homepage | Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot study [Pubmed: 32869948] |
| J Clin Hypertens | Association of urinary vanin?\1 with kidney function decline in hypertensive patients [34029440] |
| Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study [33624223] |
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