載脂蛋白B100(APOB100)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Apolipoprotein B100 (APOB100)
Apo-B100
- 編號SCA603Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長2h, 40min
- 檢測范圍5.49-4,000ng/mL
- 靈敏度最小可檢測劑量小于等于2.44ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3528 ¥ 5040 ¥ 22680 ¥ 42840 ¥ 352800
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測載脂蛋白B100(APOB100)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的載脂蛋白B100(APOB100)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 89-103 | 99 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 80-103 | 85 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的載脂蛋白B100(APOB100)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中載脂蛋白B100(APOB100)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-98% | 90-97% | 84-102% | 95-102% |
| EDTA plasma(n=5) | 80-99% | 95-102% | 81-88% | 95-105% |
| heparin plasma(n=5) | 91-99% | 90-104% | 83-97% | 88-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將載脂蛋白B100(APOB100)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的載脂蛋白B100(APOB100)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的載脂蛋白B100(APOB100)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的載脂蛋白B100(APOB100)呈正相關(guān)。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPA603Hu01 | 載脂蛋白B100(APOB100)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA603Hu01 | 載脂蛋白B100(APOB100)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA603Hu01 | 載脂蛋白B100(APOB100)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA603Hu02 | 載脂蛋白B100(APOB100)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA603Hu71 | 載脂蛋白B100(APOB100)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA603Hu23 | 載脂蛋白B100(APOB100)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA603Hu24 | 載脂蛋白B100(APOB100)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA603Hu25 | 載脂蛋白B100(APOB100)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA603Hu29 | 載脂蛋白B100(APOB100)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA603Hu27 | 載脂蛋白B100(APOB100)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA603Hu21 | 載脂蛋白B100(APOB100)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA603Hu22 | 載脂蛋白B100(APOB100)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA603Hu | 載脂蛋白B100(APOB100)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA603Hu | 載脂蛋白B100(APOB100)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA603Hu | 載脂蛋白B100(APOB100)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Toxicology | iTRAQ-based proteomic profiling of human serum reveals down-regulation of platelet basic protein and apolipoprotein B100 in patients with hematotoxicity induced by chronic occupational benzene exposure [ScienceDirect: S0300483X11004628] |
| Atherosclerosis | Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations [Pubmed:26978583] |
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509] |
| molecular nutrition & food research | Alleviating VLDL overproduction is an important mechanism for Laminaria japonica polysaccharide to inhibit atherosclerosis in LDLr-/- mice with diet-induced insulin resistance. [pubmed:27928899] |
| PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients. [pubmed:26680509] |
| Biosci Biotechnol Biochem.? | Choline and betaine ameliorate liver lipid accumulation induced by vitamin B6 deficiency in rats. [pubmed:27696964] |
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| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats [Pubmed: 32105945] |
| Ecotoxicol Environ Saf | Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen?… [Pubmed: 32911180] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| Clinical Nutrition | Postprandial dyslipidemia after a standardized high-fat meal in BMI-matched healthy individuals, and in subjects with prediabetes or Type 2 diabetes [34656950] |
| Nano Research | Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy [] |
