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乙酰膽堿(ACH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Acetylcholine (ACH)

  • 乙酰膽堿(ACH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 乙酰膽堿(ACH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對乙酰膽堿(ACH)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號 適用物種:Pan-species (General,通用) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
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LMA912Ge 乙酰膽堿(ACH)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA912Ge11 乙酰膽堿(ACH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Neurosci Bull Combined administration of D-galactose and aluminium induces Alzheimer-like lesions in brain [PubMed: 21614097]
Molecular Neurobiology Neuroprotective effects of simvastatin and Cilostazol in L-methionine-induced vascular dementia in rats [pubmed:27544235]
Turk Kardiyol Dern Ars.? Effect of statins on sirtuin 1 and endothelial nitric oxide synthase expression in young patients with a history of premature myocardial infarction [Pubmed:29664427]
JOURNAL OF LIPID RESEARCH Carotid baroreceptor stimulation in obese rats affects white and brown adipose tissues differently in metabolic protection [Pubmed: 31126973]
NEUROPHARMACOLOGY Caffeic acid phenethyl ester counteracts doxorubicin-induced chemobrain in Sprague-Dawley rats: Emphasis on the modulation of oxidative stress and?… [Pubmed: 33011510]
FASEB JOURNAL Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and?… [Pubmed: 32954574]
Exp Eye Res Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasion [Pubmed:35421396]
SAUDI J BIOL SCI Aqueous Ajwa dates seeds extract improves memory impairment in type-2 diabetes mellitus rats by reducing blood glucose levels and enhancing brain?… [Pubmed:35531250]
Arq. Bras. Med. Vet. Zootec Study on the mechanisms of Suaeda rigida polysaccharides on the heart inhibition and skeletal muscle promotion in the frog []
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