雄烯二酮(ASD)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Androstenedione (ASD)
4-Androstenedione; 4-Androstene-3,17-Dione
- 編號(hào)KSA456Ge11
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍123.5-10000pg/mL
- 可適靈敏度48.8pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)雄烯二酮(ASD)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA456Ge11 | 雄烯二酮(ASD)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA456Ge21 | 雄烯二酮(ASD)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA456Ge01 | 雄烯二酮(ASD)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA456Ge71 | 雄烯二酮(ASD)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA456Ge21 | 雄烯二酮(ASD)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA456Ge22 | 雄烯二酮(ASD)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA456Ge | 雄烯二酮(ASD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA456Ge | 雄烯二酮(ASD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA456Ge | 雄烯二酮(ASD)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA456Ge11 | 雄烯二酮(ASD)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Plos One | Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota [Pubmed:27093642] |
| Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373] |
| 33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389] |
| Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146] |
| International Journal of Biological Sciences | Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells [] |
| Molecular reproduction and development | Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries [Pubmed: 31490589] |
| DOMESTIC ANIMAL ENDOCRINOLOGY | Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells [Pubmed: 32739762] |
| ANIMAL REPRODUCTION SCIENCE | A study on steroidogenic elaborations of stroma and their regulation in response to ovarian hormones in goats [33845412] |
| Reprod Domest Anim | Extracellular Vesicles of Bovine Small Follicular Fluid Promote Ovarian Cortical Stromal Cell Proliferation and Steroidogenesis [34402549] |
| Curr Oncol | Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer [Pubmed:35323344] |
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