內皮抑素(ES)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Endostatin (ES)
- 編號KSA542Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.062ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6998 ¥ 11664 ¥ 20995 ¥ 40824 ¥ 69984
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特異性
試劑盒中的抗體對內皮抑素(ES)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
SEA542Mu | 內皮抑素(ES)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA542Mu | 內皮抑素(ES)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA542Mu01 | 內皮抑素(ES)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Cancer Immunology | Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in mice [SpringerLink: u38777121t6660l5] |
Journal of Fluoresence | Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy [SpringerLink: 4v306130302257w0] |
The Journal of Gene Medicine | Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma [Wiley: source] |
Biomedicine & Pharmacotherapy | Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma [ScienceDirect: S075333221100151X] |
Science Alert | Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source] |
The Journal of Immunology | Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast Cancer [PubMed: PMC3619527] |
Prostaglandins & Other Lipid Mediators | Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysema [Pubmed:24911647] |
American Journal of Physiology - Heart and Circulatory Physiology | Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy? [Source] |
shock | Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndrome [PubMed: 26125086] |
Clin Rev Allergy Immunol | Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature [PubMed: 26142066] |
Angiologia e Cirurgia Vascular | Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers [science:S1646706X16300052] |
Clinical Rheumatology | Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis [Pubmed:26920752] |
Blood biomarker for early blood brain barrier disruption in ischemic stroke [patent:US20170199205A1] |
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