組胺(HA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Histamine (HA)
Histamin
- 編號(hào)KSA927Ge11
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍1.23-100ng/mL
- 可適靈敏度0.52ng/mL
- 應(yīng)用Main materials for "Do It(ELISA Kit) Yourself"
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對Histamine (HA)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA927Ge41 | 組胺(HA)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA927Ge11 | 組胺(HA)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA927Ge21 | 組胺(HA)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA927Ge01 | 組胺(HA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA927Ge71 | 組胺(HA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA927Ge81 | 組胺(HA)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA927Ge21 | 組胺(HA)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA927Ge | 組胺(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA927Ge | 組胺(HA)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA927Ge | 組胺(HA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA927Ge11 | 組胺(HA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Allergy & Clinical Immunology | PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in mice [Pubmed: 21981059] |
| Asian Pacific Journal of Allergy and Immunology | Comparison of sensitization between β-lactoglobulin and its hydrolysates [Digital: 875] |
| J Chromatogr A | Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection [PubMed: 26372445] |
| Neurogastroenterology & Motility | Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea [Pubmed:26940641] |
| Italian Journal of Animal Science | Induction of immune responses and allergic reactions in piglets by injecting glycinin [doi:1828051X.2016.1144488] |
| Int J Clin Exp Pathol | Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism [files:ijcep0019305.pdf] |
| PLoS One. | Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin [pmc:PMC4734762] |
| Int Immunopharmacol. | Evaluation of anaphylactoid constituents in vitro and in vivo. [pubmed:27984711] |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349] |
| INTERNATIONAL JOURNAL OF MOLECULAR?SCIENCES? | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
| Royal?Society?Open?Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
| International?Journal?of?Pharmaceutical?Sciences?and?Research | MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL [] |
| Allergy | Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial [Pubmed: 32030780] |
| MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
| Nature Communications | A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752] |
| CCR3?shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells [Pubmed: 32742345] | |
| ANALYTICA CHIMICA ACTA | Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of?… [Pubmed: 32892931] |
| Annals of Tropical Medicine & Public Health | Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq [] |
| pharmacol res& perspect | Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs [34014033] |
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