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層粘連蛋白(LN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Laminin (LN)

LAM

  • 層粘連蛋白(LN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 層粘連蛋白(LN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對層粘連蛋白(LN)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658]
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592]
Clin Exp Metastasis. Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression [Pubmed:24807734]
European Journal of Obstetrics & Gynecology and Reproductive Biology Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis. [Pubmed:24813083]
Archives of Plastic Surgery Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas [PubMed: 26430624]
Canadian Journal of Physiology and Pharmacology Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells [Doi: Abs]
MOLECULAR AND CLINICAL ONCOLOGY Clinical significance of serum laminin and typea??IV collagen levels in cutaneous melanoma patients [Pubmed:27330797]
Am J Transplant. Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation. [pubmed:27456745]
Asian Pacific Journal of Cancer Prevention Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response [pubmed:29172262]
Diabetes Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes [Pubmed:29986925]
European Food Research and Technology Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis [10.1007:s00217-018-3119-z]
Experimental Lung Research High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects [Pubmed: 31290711]
Neurotherapeutics Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair [Pubmed: 31758411]
Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellul?rmatrix von Kalbs-Stimmlippen []
ARCHIV?EUROMEDICA CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE []
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