硝基酪氨酸(NT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Nitrotyrosine (NT)
3-Nitro-L-Tyrosine; 3-Nitrotyrosine
- 編號KSB863Ge11
- 物種Pan-species (General,通用) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍9.88-800ng/mL
- 可適靈敏度3.95ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 8726 ¥ 14544 ¥ 26179 ¥ 50904 ¥ 87264
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特異性
試劑盒中的抗體對硝基酪氨酸(NT)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Pan-species (General,通用) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
CPB863Ge11 | 硝基酪氨酸(NT)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPB863Ge21 | 硝基酪氨酸(NT)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAB863Ge01 | 硝基酪氨酸(NT)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEB863Ge | 硝基酪氨酸(NT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEB863Ge | 硝基酪氨酸(NT)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB863Ge | 硝基酪氨酸(NT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB863Ge11 | 硝基酪氨酸(NT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Molecular Medicine Reports | 3-Nitrotyrosine, a biomarker for cardiomyocyte apoptosis induced by diabetic cardiomyopathy in a rat model [Pubmed: 23970331] |
Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835] |
J Histochem Cytochem | Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats [PubMed: 26385569] |
canadian journal of physiology and pharmacology | Protective effects of Telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuro-inflammation and amyloidogenesis: possible role of brain derived neurotrophic factor [10.1139:cjpp-2017-0042] |
Orphanet journal of rare diseases | The cardiovascular phenotype of adult patients with phenylketonuria [Pubmed: 31492166] |
ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990] |
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