三碘甲狀腺原氨酸(T3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Triiodothyronine (T3)
3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin
- 編號(hào)KSA453Ge11
- 物種Pan-species (General,通用) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍123.5-10,000pg/mL
- 可適靈敏度51.7pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 5875 ¥ 9792 ¥ 17626 ¥ 34272 ¥ 58752
- 欲了解實(shí)際交易價(jià)格和更多情況,請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
特異性
試劑盒中的抗體對三碘甲狀腺原氨酸(T3)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Pan-species (General,通用) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
CPA453Ge11 | 三碘甲狀腺原氨酸(T3)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA453Ge21 | 三碘甲狀腺原氨酸(T3)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA453Ge01 | 三碘甲狀腺原氨酸(T3)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA453Ge71 | 三碘甲狀腺原氨酸(T3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA453Ge21 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | WB; IHC; ICC; IP. |
MAA453Ge23 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA453Ge22 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA453Ge24 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA453Ge25 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA453Ge27 | 三碘甲狀腺原氨酸(T3)單克隆抗體 | WB; IHC; ICC; IP. |
LAA453Ge72 | 三碘甲狀腺原氨酸(T3)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
CEA453Ge | 三碘甲狀腺原氨酸(T3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
IEA453Ge | 三碘甲狀腺原氨酸(T3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA453Ge | 三碘甲狀腺原氨酸(T3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA453Ge | 抗三碘甲狀腺原氨酸抗體(Anti-T3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
CCA453Ge | 三碘甲狀腺原氨酸(T3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA453Ge | 三碘甲狀腺原氨酸(T3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA453Ge11 | 三碘甲狀腺原氨酸(T3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Aquatic Toxicology | Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae [ScienceDirect: S0166445X12000215] |
Aquatic Toxicology | Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis [PubMed: 23220413] |
European Journal of Zoological Research | Effect of dietary tryptophan on plasma growth hormone and thyroid hormone in broiler chicks [Scholarsresearchlibrary: Source] |
Life Science Journal | Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies [Lifesciencesite: Source] |
PLoS One | A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism [Pubmed: 23468891] |
Aquat Toxicol | Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. [ScienceDirect: S0166445X13002129] |
Aquatic Toxicology | Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae [Pubmed:25456233] |
Chemosphere | Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam?Ruditapes philippinarum [science:S0045653516302016] |
Toxicological?Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267] |
Aquat Toxicol. | Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). [pubmed:28187359] |
Bulletin of the World Health Organization | Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders [handle:123456789] |
Biological Rhythm Research | Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes [09291016.2016.1275393] |
Toxicological Sciences | Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666] |
Environmental Pollution | Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead [10.1016/j.envpol.2017.06.053] |
Sciencepaper online | 四溴雙酚A對菲律賓蛤仔的生長抑制效應(yīng)機(jī)理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum [5790267088906950000] |
Tropical Journal of Pharmaceutical Research | Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats [10.4314/tjpr.v16i5.9] |
s-space | Screening thyroid hormone disrupting effects of benzophenones using GH3, FRTL-5 cells and zebrafish [10371/137708] |
s-space | Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult [10371/137708] |
Environmental Pollution | Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. [pubmed:28624628] |
Environmental?Science?& Technology | Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish [Pubmed:29995391] |
Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
Toxicological?Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
Drug?metabolism and disposition | Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liver [Pubmed:29555828] |
Archives?Animal?Breeding | Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes [10.5194:aab-61-191-2018] |
Indian Journal of Animal Sciences | Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition [:] |
Environmental Pollution | Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspring [Pubmed: 30243188] |
Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008] |
Drug Metabolism and Disposition | Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing?… [Pubmed: 30518657] |
Science?of?the?Total?Environment | Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafish [Doi: 10.1016/j.scitotenv.2019.02.198] |
Theriogenology? | Thyroid hormone profiles and TSH evaluation during early pregnancy and the transition period in dairy cows [Pubmed: 30784791] |
Chemosphere | Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPA [Pubmed: 30844590] |
Aquatic Toxicology | Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevis [Pubmed: 31276910] |
ecotoxicology and environmental safety | Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae [Pubmed: 31539807] |
Cells | MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model [Pubmed: 31540304] |
Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
Autoimmunity | Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balance [Pubmed: 31793369] |
AQUATIC TOXICOLOGY | Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryos [Pubmed: 32172180] |
Chemosphere | Effect of triadimefon and its metabolite on adult amphibians Xenopus laevis [Pubmed: 31743868] |
Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis [Pubmed: 33177714] |
Toxicol Lett | Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio) [Pubmed: 32525014] |
Ecotoxicology | Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlor [Pubmed: 32468518] |
Brazilian Journal of Development | Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposi??o materna ao triclosan causa?… [] |
CHEMOSPHERE | Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio) [] |
Andrologia | Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine?\treated rat dams during pregnancy and lactation [33433934] |
The Journal of Clinical Endocrinology & Metabolism | High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression [33758937] |
Endocrinology | Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism [34019632] |
Ecotoxicol Environ Saf | Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development [34126306] |
Journal of Hazardous Materials | Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect [33647619] |
Pharmaceutics | Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays [] |
Pharmaceutics | The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver [34683936] |
Aquat Toxicol | Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations [34933138] |
Environmental Pollution | Probiotics inhibit the stunted growth defect of perfluorobutanesulfonate via stress and thyroid axes in zebrafish larvae [34428700] |
Ecotoxicol Environ Saf | Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae [Pubmed:35131581] |
Toxics | Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio) [Pubmed:35448459] |
留言咨詢