胰蛋白酶原激活肽(TAP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP)
- 編號KSA634Hu11
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍123.5-10000pg/mL
- 可適靈敏度54.1pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
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特異性
試劑盒中的抗體對胰蛋白酶原激活肽(TAP)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA634Hu01 | 胰蛋白酶原激活肽(TAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA634Hu21 | 胰蛋白酶原激活肽(TAP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA634Hu11 | 胰蛋白酶原激活肽(TAP)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA634Hu01 | 胰蛋白酶原激活肽(TAP)多克隆抗體 | WB; IHC; ICC; IP. |
LAA634Hu71 | 胰蛋白酶原激活肽(TAP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAA634Hu21 | 胰蛋白酶原激活肽(TAP)單克隆抗體 | WB; IHC; ICC; IP. |
CEA634Hu | 胰蛋白酶原激活肽(TAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA634Hu | 胰蛋白酶原激活肽(TAP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA634Hu11 | 胰蛋白酶原激活肽(TAP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Diabetologia | Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice [SpringerLink: x4l01435t34452jr] |
Molecular Biology Reports | Prior peritoneal lavage with hot 0.9% saline induces HSP70 expression and protects against cerulein-induced acute pancreatitis in rats [Pubmed: 23096089] |
Journal of the Pancreas | Case report of mannose-binding lectin (MBL) deficiency and postoperative sepsis and coagulopathy in a patient following total pancreatectomy for chronic pancreatitis. [Pubmed:25262717] |
Journal of Gastroenterology | A small molecule inhibitor of NFκB blocks ER stress and the NLRP3 inflammasome and prevents progression of pancreatitis [pubmed:27418337] |
Indian Journal of Pharmaceutical Sciences | Effects of Calcium Channel Blockers on Trypsinogen Activation and Severity of Cerulein-induced Acute Pancreatitis in Rats [IJPS:Source] |
Tropical Journal of Pharmaceutical Research | Investigation of non-pharmaceutical cures for acute pancreatitis induced by cerulein in rats [] |
Pancreas | The Regulatory Effect of the Kinase Inhibitor PD98059 on Autophagic Flux During Trypsinogen Activation in Pancreatic Acinar Cells [Pubmed: 32011537] |
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