抗γ-氨基丁酸抗體(Anti-gABA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Anti-Gamma-Aminobutyric Acid Antibody (Anti-gABA)
4-Aminobutyric Acid
- 編號AEA900Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法間接法
- 反應時長2h, 30min
- 檢測范圍3.12-200ng/mL
- 靈敏度最小可檢測劑量小于等于1.18ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3780 ¥ 5400 ¥ 24300 ¥ 45900 ¥ 378000
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測抗γ-氨基丁酸抗體(Anti-gABA)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的抗γ-氨基丁酸抗體(Anti-gABA)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-105 | 101 |
| EDTA plasma(n=5) | 84-91 | 87 |
| heparin plasma(n=5) | 80-96 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的抗γ-氨基丁酸抗體(Anti-gABA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中抗γ-氨基丁酸抗體(Anti-gABA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 81-93% | 94-102% | 80-101% |
| EDTA plasma(n=5) | 91-102% | 92-99% | 80-95% | 92-101% |
| heparin plasma(n=5) | 86-93% | 92-101% | 96-103% | 87-103% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分鐘；
6. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將抗原包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的抗γ-氨基丁酸抗體(Anti-gABA)與連接于固相載體上的抗原結合，然后加入HRP標記的二抗，徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的抗γ-氨基丁酸抗體(Anti-gABA)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關產品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPA900Ge11 | γ-氨基丁酸(gABA)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge21 | γ-氨基丁酸(gABA)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge41 | γ-氨基丁酸(gABA)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA900Ge01 | γ-氨基丁酸(gABA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA900Ge02 | γ-氨基丁酸(gABA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA900Ge71 | γ-氨基丁酸(gABA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA900Ge81 | γ-氨基丁酸(gABA)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| CEA900Ge | γ-氨基丁酸(gABA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA900Ge | 抗γ-氨基丁酸抗體(Anti-gABA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA900Ge | γ-氨基丁酸(gABA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA900Ge11 | γ-氨基丁酸(gABA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Toxicology and Industrial Health | Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum [PubMed: 21444355] |
| Neurosci Lett | Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear. [PubMed: 18577424] |
| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
| Scientific Reports | Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension [pubmed:27917928] |
| Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats [Pubmed:28861894] |
| Scientific Reports | A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme [Pubmed: 30341396] |
| Neurochemical?Research | Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission [Pubmed: 30666488] |
| Neuroscience?Letters | Differentiation of human glioblastoma U87 cells into cholinergic neuron [Pubmed: 30928478] |
| Neurochemical Journal | Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism [] |
| Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats. [] | |
| Experimental and Therapeutic Medicine | Antiepileptic effects of exogenous β?hydroxybutyrate on kainic acid?induced epilepsy [Pubmed: 33101467] |
| Exp Anim | Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation [33828024] |
| Neurogenetics | Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases [34101060] |
| Sci Rep | Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission [34475508] |
| J Food Biochem | Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies [Pubmed:35102569] |
