和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Copeptin (CPP)
C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin
- 編號SEA365Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.9pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2856 ¥ 4080 ¥ 18360 ¥ 34680 ¥ 285600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測和肽素(CPP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的和肽素(CPP)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 89-103 | 98 |
| heparin plasma(n=5) | 81-97 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的和肽素(CPP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中和肽素(CPP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-94% | 79-101% | 92-101% | 81-90% |
| EDTA plasma(n=5) | 96-105% | 85-101% | 80-103% | 97-104% |
| heparin plasma(n=5) | 89-96% | 88-103% | 87-101% | 89-99% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將和肽素(CPP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的和肽素(CPP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的和肽素(CPP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的和肽素(CPP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA365Hu01 | 和肽素(CPP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA365Hu31 | 和肽素(CPP)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA365Hu22 | 和肽素(CPP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA365Hu21 | 和肽素(CPP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA365Hu01 | 和肽素(CPP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA365Hu08 | 和肽素(CPP)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA365Hu28 | 和肽素(CPP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA365Hu | 和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA365Hu | 和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA365Hu | 和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA365Hu | 和肽素(CPP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| International Journal of Clinical and Experimental Medicine | Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic … [files:ijcem0031358.pdf] |
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| Hindawi Publishing Corporation | Early Prediction of Preeclampsia [journals:297397] |
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| American Journal of Physiology-Renal?Physiology | Experimental Heat Stress Nephropathy and Liver Injury are Improved by Allopurinol [Pubmed:29667911] |
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| 中國病理生理雜志 | 和肽素水平對大鼠心腎綜合征的早期預(yù)測價值 [filename:ZBLS201802013] |
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| experimental physiology | Cognitive performance is associated with cerebral oxygenation and peripheral oxygen saturation, but not plasma catecholamines, during graded normobaric hypoxia [] |
| international urology and nephrology | Dialysate copeptin and peritoneal transport in incident peritoneal dialysis patients [Pubmed: 31187425] |
| American Journal of Physiology-renal Physiology | The contribution of collecting duct NOS1 to the concentrating mechanisms in male and female mice [Pubmed: 31241990] |
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| Scientific Reports | Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion [Pubmed: 31863073] |
| BMC Nephrology | Copeptin is independently associated with vascular calcification in chronic kidney disease stage 5 [Pubmed: 32033584] |
| Archives of Biochemistry and Biophysics | Micro RNA 146a gene variant/TNF-α/IL-6/IL-1 β; A cross-link axis inbetween oxidative stress, endothelial dysfunction and neuro-inflammation in acute ischemic stroke … [Pubmed: 31738890] |
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| 河北醫(yī)科大學(xué)學(xué)報 | 重組人腦利鈉肽治療慢性肺心病急性加重期的臨床效果及對5-HT 濃度的影響 [] |
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| International Journal of Women?ˉs Health and Reproduction Sciences | Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women? [] |
| Dicle T?p Dergisi | Copeptin levels are more elevated and associated with cardiovascular events in the unity of chronic ischemic heart and chronic kidney diseases [] |
| High serum copeptin may be a marker of an increased carotid intima-media thickness in asymptomatic patients with type 1 diabetes. [] | |
| Nutrients | The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose [] |
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