D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for D-Dimer (D2D)
D 2 Dimer
- 編號CEA506Eq
- 物種Equus caballus; Equine (Horse，馬) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍617.3-50,000pg/mL
- 靈敏度最小可檢測劑量小于等于283.1pg/mL.
- 樣本類型plasma
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3251 ¥ 4644 ¥ 20898 ¥ 39474 ¥ 325080
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測D-二聚體(D2D)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的D-二聚體(D2D)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| sodium citrate plasma(n=5) | 87-102 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的D-二聚體(D2D)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中D-二聚體(D2D)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| sodium citrate plasma(n=5) | 89-98% | 80-94% | 93-101% | 88-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將D-二聚體(D2D)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Equus caballus; Equine (Horse，馬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CEA506Eq | D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA506Eq | D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| 22 | Determinants of the thrombogenic potential of multiwalled carbon nanotubes [ScienceDirect: S0142961211004819] |
| Journal of Thrombosis and Haemostasis | Exogenous Activated Protein C Inhibits the Progression of Diabetic Nephropathy [Pubmed: 22236035] |
| PLOS ONE | Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice [PlosOne: Source] |
| Haemetology | Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India [Pubmed: 23992124] |
| Journal of Trauma and Acute Care Surgery | Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine model [Lww:Source] |
| Journal of Thrombosis and Haemostasis | Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells [Pubmed:25399514] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
| Medical Science Monitor | Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic Coagulopathy [Pubmed:25676919] |
| Blood | Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation. [PubMed: 25575539] |
| Am J Physiol Regul Integr Comp Physiol | Procoagulant and Fibrinolytic Activity after Polytrauma in Rat [PubMed: 26632604] |
| hypertension Research | Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat model [PubMed: 26155749] |
| Molecular Nutrition & Food Research | Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502] |
| JCI Insight. | KLF2 and KLF4 control endothelial identity and vascular integrity. [pubmed:28239661] |
| The American Society of Anesthesiologists | Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathy [pubmed:27841821] |
| 32 | Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target [pubmed:28289017] |
| The American Journal of Pathology | Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulin [pubmed:28739343] |
| Atherosclerosis | Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin. [pubmed:28923781] |
| 28 | Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Mice [pubmed:29162596] |
| Journal of Thrombosis and Thrombolysis | Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo. [pubmed:28417266] |
| Journal?of?Investigative?Surgery | Experimental Evaluation of a New Tissue Factor-Based Topical Hemostat (TT-173) for Treatment of Hepatic Bleeding [Pubmed: 30380352] |
| Marine Drugs | The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230] |
| Intensive Care Med Exp | Complement factor D is linked to platelet activation in human and rodent sepsis [34396466] |
| Thromb Res | NLRP3 Inflammasome contributes to endotoxin-induced coagulation [Pubmed:35421682] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
