血管緊張素Ⅱ(AngII)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Angiotensin II (AngII)
Ang-II; Angiotensin-2
- 編號CEA005Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍24.69-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于9.44pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測血管緊張素Ⅱ(AngII)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的血管緊張素Ⅱ(AngII)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-97 | 92 |
| EDTA plasma(n=5) | 94-101 | 97 |
| heparin plasma(n=5) | 98-105 | 102 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管緊張素Ⅱ(AngII)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管緊張素Ⅱ(AngII)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 97-105% | 97-104% | 80-89% |
| EDTA plasma(n=5) | 92-101% | 98-105% | 79-103% | 81-94% |
| heparin plasma(n=5) | 89-103% | 79-101% | 92-101% | 94-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準品、試劑及樣本的準備；
2. 加樣（標(biāo)準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將血管緊張素Ⅱ(AngII)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA005Hu21 | 血管緊張素Ⅱ(AngII)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA005Hu01 | 血管緊張素Ⅱ(AngII)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA005Hu81 | 血管緊張素Ⅱ(AngII)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA005Hu71 | 血管緊張素Ⅱ(AngII)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA005Hu22 | 血管緊張素Ⅱ(AngII)單克隆抗體 | WB; IHC; ICC; IP. |
| CEA005Hu | 血管緊張素Ⅱ(AngII)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA005Hu | 血管緊張素Ⅱ(AngII)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA005Hu | 血管緊張素Ⅱ(AngII)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA005Hu11 | 血管緊張素Ⅱ(AngII)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Nature Medicine | Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition [MolMed: 10_11_liu] |
| The European Journal of Clinical Investigation | Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes [Wiley: source] |
| Immunopharmacology and Inflammation | Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid [ScienceDirect: S0188440910001049] |
| Nephrology | Effect of aggregated immunoglobulin A1 from immunoglobulin A nephropathy patients on nephrin expression in podocytes [Wiley: source] |
| PLoS One | Angiotensin II and Angiotensin-(1-7) in Paraventricular Nucleus Modulate Cardiac Sympathetic Afferent Reflex in Renovascular Hypertensive Rats [PubMed: PMC3527547] |
| BioMed Research International | Heterogeneous Downregulation of Angiotensin II AT1-A and AT1-B Receptors in Arterioles in STZ-Induced Diabetic Rat Kidneys [Hindawi: 947506] |
| 34 | Biglcan is beneficial in angiotensin II induced heart failure by preventing cardiac inflammation and remodeling improving LV function and mortality by preventing transdifferentiation of myofibroblasts [Oxfordjournals: Source] |
| Pflügers Archiv-European Journal of Physiology | SOD1 gene transfer into paraventricular nucleus attenuates hypertension and sympathetic activity in spontaneously hypertensive rats [Pubmed: 23114721] |
| Eur J Pharmacol. | Angiotensin II and vasopressin are involved in the defense system against anaphylactic hypotension in anesthetized rats [Pubmed:24650734] |
| J Mol Cell Cardiol. | Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction [Pubmed:25871829] |
| Mol Med Rep. | High glucose induces activation of the local renin?angiotensin system in glomerular endothelial cells [Pubmed:24337709] |
| Mol Med Rep. | Angiotensin II promotes the progression of human gastric cancer [Pubmed:24424956] |
| PLOS ONE | Intermedin in the Paraventricular Nucleus Attenuates Cardiac Sympathetic Afferent Reflex in Chronic Heart Failure Rats [Pubmed:24709972] |
| Journal of Vascular Surgery | Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606] |
| Biochim Biophys Acta. | Cathepsin G activity lowers plasma LDL and reduces atherosclerosis [Pubmed:25092171] |
| Acta Physiologica | Superoxide anions in the paraventricular nucleus mediate cardiac sympathetic afferent reflex in insulin resistance rats [Pubmed:25307720] |
| Neuroscience Letters | Administration of exogenous 1,25(OH)2D3 normalizes overactivation of the central renin-angiotensin system in 1α(OH)ase knockout mice [Pubmed:25576706] |
| 34 | A background Ca2+?entry pathway mediated by TRPC1/TRPC4 is critical for development of pathological cardiac remodelling [PubMed: 26069213] |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca2 [PubMed: 26706290] |
| Physiological Research | Renal Sympathetic Denervation Improves Cardiac Dysfunction [PubMed: 25804096] |
| Nutrition | Effects of Rubus occidentalis extract on blood pressure in subjects with prehypertension: randomized, double-blinded, placebo controlled clinical trial [PubMed: 26740254] |
| J Am Soc Hypertens | Angiotensin-(1–7) enhances the effects of angiotensin II on the cardiac sympathetic afferent reflex and sympathetic activity in rostral ventrolateral medulla in renovascular hypertensive rats [PubMed: 26428223] |
| PLoS One. | Up ReCavia (Guinea pig )lation of cystathione γ lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol–Caffeine Induced Left Ventricular Hypertrophy in Wistar Kyoto Rats [Pubmed:26963622] |
| Nutrition | Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial [Pubmed:26740254] |
| Journal of Atherosclerosis and Thrombosis | Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase ReCavia (Guinea pig )lation in Human Carotid Atheroma in vitro [Pubmed:26947595] |
| Mol Neurobiol | A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats [Pubmed:26867655] |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro [Pubmed:26706290] |
| PLoS One. | Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. [pubmed:27880816] |
| Oxidative Medicine and Cellular Longevity | Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice [pubmed:27698952] |
| Journal of the Renin-Angiotensin-Aldosterone System | Angiotensin type 2 receptor null mice express reduced levels of renal angiotensin converting enzyme-2/angiotensin (1-7)/Mas receptor and exhibit greater high-fat diet-induced kidney injury [1470320316661871.abstract] |
| Microvascular?Research | Pleiotropic?effects?of?survivin?in?vascular endothelial cells. [pubmed:27373518] |
| PLOS?ONE | Reduced?Expression?of the?Extracellular?Calcium-Sensing?Receptor?(CaSR) Is?Associated?withActivation?of the?Renin-Angiotensin?System?(RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. [pubmed:27391973] |
| journal of pharmacological sciences | β-Aminoisobutyric?acid?ameliorates?the?renal?fibrosis?in?mouse?obstructed?kidneys?via?inhibitionof?renal?fibroblast?activation?and?fibrosis. [pubmed:28433566] |
| Experimental?Animals | Ginsenoside?Rb1?improves?cardiac?function?and?remodeling?in?heart?failure. [pubmed:28367863] |
| Oncotarget | Effects of renal denervation on monocrotaline induced pulmonary remodeling [pubmed:28187460] |
| The American Journal of Pathology | The Renin-Angiotensin System Regulates Neurodegeneration in a Mouse Model of Optic Neuritis [pubmed:28919108] |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788] |
| Frontiers in Physiology | Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression [pubmed:29066981] |
| Neurotherapeutics | Brain Renin–Angiotensin System Blockade Attenuates Methamphetamine-Induced Hyperlocomotion and Neurotoxicity [Pubmed:29464572] |
| International Journal of Cardiology | ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in?… [Pubmed:29506687] |
| Neuroscience | Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats [Pubmed:29684507] |
| American Journal of Physiology-Renal?Physiology | Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats [Pubmed:29846109] |
| Journal of the American Society of Hypertension | Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system [Pubmed:29752040] |
| Evidence-based?Complementary and Alternative?Medicine? | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313] |
| Brazilian Journal of Medical and Biological Research | Renovascular hypertension increases serum TNF and CX3CL1 in experimental Trypanosoma cruzi infection [Pubmed:29590257] |
| American Journal of Physiology-Heart?and Circulatory Physiology | Sex Differences in the Central and Peripheral Manifestations of Ischemia-induced Heart Failure in Rats [Pubmed: 30289294] |
| Toxicology Mechanisms and?Methods | Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats [Pubmed: 30426860] |
| Journal of?Clinical?and Aesthetic Dermatology | Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar. [] |
| International Journal of Ophthalmology | Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetes [Pubmed: 30588418] |
| JOURNAL OF?STRENGTH?AND CONDITIONING RESEARCH | ACE Gene Plays a Key Role in Reducing Blood Pressure in The Hyperintensive Elderly After Resistance Training [Pubmed: 30908459] |
| The?American?Journal?of?the?Medical?Sciences | T lymphocyte-based renin angiotensin system in obesity [] |
| Journal of Alzheimers Disease | Cerebrospinal Fluid Changes in the Renin-Angiotensin System in Alzheimer's Disease [Pubmed: 31594235] |
| Andrologia | Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status [Pubmed: 31989676] |
| Molecular Medicine Reports | BPA disrupts 17?estradiol?mediated hepatic protection against ischemia/reperfusion injury in rat liver by upregulating the Ang II/AT1R signaling pathway [Pubmed: 32319667] |
| MOLECULAR MEDICINE REPORTS | The sympathetic transmitter norepinephrine inhibits VSMC proliferation induced by TGFβ by suppressing the expression of the TGFβ receptor ALK5 in aorta?… [Pubmed: 32319652] |
| Cell Mol Neurobiol | Predator Scent-Induced Sensitization of Hypertension and Anxiety-like Behaviors [Pubmed: 33201417] |
| biochemistry biophysical reports | Effect of repetitive potassium iodide on thyroid and cardiovascular functions in elderly rats [Pubmed: 33024842] |
| bioscience reports | Angiotensin II enhances the proliferation of Natural Killer/T-cell lymphoma cells via activating PI3K/Akt signaling pathway [Pubmed: 32969473] |
| AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | A (pro) renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting intrarenal renin-angiotensin system [Pubmed: 32865014] |
| Acta Endocrinologica-Bucharest | THE ROLE OF HIGH FOLLICULAR LEVELS OF ANGIOTENSIN II AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN ANTICIPATING THE?… [Pubmed: 32685035] |
| JOURNAL OF PROTEOME RESEARCH | Proteomics and informatics for understanding phases and identifying biomarkers in COVID-19 disease [Pubmed: 32657586] |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
| BIOMEDICINE & PHARMACOTHERAPY | Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats [Pubmed: 32570115] |
| CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats [Pubmed: 32349626] |
| Evidence-Based Complementary and Alternative Medicine | Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats [Pubmed: 33133215] |
| Oxidative Medicine and Cellular Longevity | Sinapic Acid Attenuates Cardiovascular Disorders in Rats by Modulating Reactive Oxygen Species and Angiotensin Receptor Expression [Pubmed: 32765804] |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats [] |
| Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?… [] | |
| Physiol Behav | Low-load resistance training with blood flow restriction prevent renal function decline: The role of the redox balance, angiotensin 1-7 and vasopressin [33340514] |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990] |
| Int J Hypertens | The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models [33884205] |
| Physiol Rep | (Pro) renin receptor antagonist PRO20 attenuates nephrectomy?\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/|??\catenin signaling [34057312] |
| Redox Biology | Renovascular effects of inorganic nitrate following ischemia-reperfusion of the kidney [33360353] |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease [33995926] |
| researchsquare | Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice [] |
| Molecular Medicine Reports | Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats [34278476] |
| BMC Musculoskeletal Disorders | Ang-(1–7) protects skeletal muscle function in aged mice [34548056] |
| J Am Heart Assoc | Maternal Angiotensin II–Induced Hypertension Sensitizes Postweaning High‐Fat Diet–Elicited Hypertensive Response Through Increased Brain Reactivity in Rat?… [34482712] |
| Biomed Pharmacother | Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti?… [34736077] |
| J Am Heart Assoc | Loss of the Protective Effect of Estrogen Contributes to Maternal Gestational Hypertension‐Induced Hypertensive Response Sensitization Elicited by Postweaning?… [Pubmed:35014859] |
| J Gerontol A Biol Sci Med Sci | Dysregulation of ACE-1 in normal aging and the early stages of Alzheimer's disease [Pubmed:35396835] |
| Biomedicine & Pharmacotherapy | Corynoline protects ang II-induced hypertensive heart failure by increasing PPARα and Inhibiting NF-κB pathway [Pubmed:35658238] |
留言咨詢
