羥脯氨酸(Hyp)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Hydroxyproline (Hyp)
(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)
- 編號CEA621Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍61.7-5,000ng/mL
- 靈敏度最小可檢測劑量小于等于28.5ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3342 ¥ 4774 ¥ 21481 ¥ 40576 ¥ 334152
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測羥脯氨酸(Hyp)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的羥脯氨酸(Hyp)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-99 | 94 |
| EDTA plasma(n=5) | 85-98 | 90 |
| heparin plasma(n=5) | 91-105 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的羥脯氨酸(Hyp)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中羥脯氨酸(Hyp)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-104% | 97-105% | 93-101% | 93-105% |
| EDTA plasma(n=5) | 87-95% | 97-105% | 78-89% | 84-101% |
| heparin plasma(n=5) | 83-94% | 85-93% | 79-88% | 78-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將羥脯氨酸(Hyp)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA621Ge11 | 羥脯氨酸(Hyp)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA621Ge21 | 羥脯氨酸(Hyp)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA621Ge01 | 羥脯氨酸(Hyp)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA621Ge | 羥脯氨酸(Hyp)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA621Ge | 羥脯氨酸(Hyp)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA621Ge | 羥脯氨酸(Hyp)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA621Ge11 | 羥脯氨酸(Hyp)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
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| Life Sciences | Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in rats [PubMed: 26165752] |
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| Life?Sciences | Celecoxib?attenuates?depressive-like?behavior?associated?with?immunological?liver?injury?inC57BL/6?mice?through TNF-α and NF-κb?dependent?mechanisms. [pubmed:27580523] |
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| Journal of?Cellular?Biochemistry | Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits [Pubmed:29932250] |
| Canadian Journal of Physiology and Pharmacology | Renin–angiotensin system inhibition ameliorates CCl-induced liver fibrosis in mice through the inactivation of nuclear transcription factor kappa B [10.1139:cjpp-2017-0728] |
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| Cell Death & Disease | Therapeutic effect of hepatocyte growth factor-overexpressing bone marrow-derived mesenchymal stem cells on CCl 4-induced hepatocirrhosis. [] |
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| Annals of Surgical Treatment and Research | Is platelet-rich plasma improves the anastomotic healing in hyperthermic intraperitoneal chemotherapy with oxaliplatin: an experimental rat study [Pubmed: 32051817] |
| Arch Med Res | Protective Effects of Contractubex? on Stricture Formation after Experimental Corrosive Esophageal Burns in Rats [Pubmed: 32654881] |
| International Medical Journal? | Effect of Oral Channa striatus in Laparotomy Wound Healing of Malnourished Rats. [] |
| Dis Marker | Handgrip Strength and Vitamin D as Predictors of Liver Fibrosis and Malnutrition in Chronic Hepatitis C Patients [33884041] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| Front Cell Dev Biol | Curdione and Schisandrin C Synergistically Reverse Hepatic Fibrosis via Modulating the TGF-β Pathway and Inhibiting Oxidative Stress [34858986] |
