氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)
NT-Pro-BNP; ; N-BNP
- 編號SEA485Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.056ng/mL.
- 樣本類型serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3696 ¥ 5280 ¥ 23760 ¥ 44880 ¥ 369600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測氨基端前腦鈉素(NT-ProBNP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的氨基端前腦鈉素(NT-ProBNP)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-93 | 84 |
| EDTA plasma(n=5) | 79-99 | 85 |
| heparin plasma(n=5) | 83-90 | 86 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的氨基端前腦鈉素(NT-ProBNP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中氨基端前腦鈉素(NT-ProBNP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-98% | 95-103% | 95-103% | 90-98% |
| EDTA plasma(n=5) | 81-95% | 80-99% | 83-104% | 88-102% |
| heparin plasma(n=5) | 92-101% | 82-97% | 89-99% | 97-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將氨基端前腦鈉素(NT-ProBNP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的氨基端前腦鈉素(NT-ProBNP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的氨基端前腦鈉素(NT-ProBNP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的氨基端前腦鈉素(NT-ProBNP)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA485Ra01 | 氨基端前腦鈉素(NT-ProBNP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA485Ra21 | 氨基端前腦鈉素(NT-ProBNP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA485Ra22 | 氨基端前腦鈉素(NT-ProBNP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA485Ra31 | 氨基端前腦鈉素(NT-ProBNP)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA485Ra01 | 氨基端前腦鈉素(NT-ProBNP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA485Ra08 | 氨基端前腦鈉素(NT-ProBNP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA485Ra79 | 氨基端前腦鈉素(NT-ProBNP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA485Ra28 | 氨基端前腦鈉素(NT-ProBNP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA485Ra | 氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA485Ra | 氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA485Ra | 氨基端前腦鈉素(NT-ProBNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA485Ra | 氨基端前腦鈉素(NT-ProBNP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Eur Rev Med Pharmacol Sci. | The role of serum n-terminal pro-brain natriuretic peptide in transient tachypnea of the newborn [Europeanreview: Source] |
| Transplantation Proceedings | Vascular Adhesion Protein-1, a Novel Molecule, in Kidney and Heart Allograft Recipients [Pubmed: 23769096] |
| 34 | Paradoxical low-flow, low-gradient aortic stenosis despite preserved left ventricular ejection fraction: new insights from weights of operatively excised aortic valves [Pubmed: 24755006] |
| Integrative Medicine Insights | A Randomized Controlled Trial to Study the Effect of Yoga Therapy on Cardiac Function and N Terminal Pro BNP in Heart Failure [NCBI: PMC3981569] |
| Liver Int.? | Diabetes enhances the intrahepatic vascular response to endothelin-1 in cirrhotic rats: association with the ETA receptor and pERK up-regulation. [Pubmed:24636620] |
| Eur J Heart Fail. | Growth-differentiation factor 15 and osteoprotegerin in acute myocardial infarction complicated by cardiogenic shock: a biomarker substudy of the IABP-SHOCK II-trial. [Pubmed:24903195] |
| Scientific Reports | Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension [PubMed: 25747153] |
| Int J Mol Sci | The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension [PubMed: 25984599] |
| scientific reports | Effect of Treatment on Body Fluid in Patients with Unilateral Aldosterone Producing Adenoma: Adrenalectomy versus Spironolactone [PubMed: 26477337] |
| Liver Int | Diabetes enhances the intrahepatic vascular response to endothelin‐1 in cirrhotic rats: association with the ETA receptor and pERK up‐regulation [PubMed: 24636620] |
| Int J Clin Exp Pathol | Qiliqiangxin improves cardiac function and attenuates cardiac remodeling in rats with experimental myocardial infarction [PubMed: 26261541] |
| Iranian Journal of Pediatrics | NT-proBNP in Children With Left to Right Shunt and Dilated Cardiomyopathy [serve:4485] |
| International Journal of Cardiology | Human myoblast transplantation in mice infarcted heart alters the expression profile of cardiac genes associated with left ventricle remodeling [Pubmed:26457413] |
| IJC Heart & Vasculature | Cardiovascular changes in patients with non-severe Plasmodium vivax malaria [article:S2352-9067(16)30010-0] |
| Heart Lung Circ | Soluble Glycoprotein 130 and Heat Shock Protein 27 as Novel Candidate Biomarkers of Chronic Heart Failure with Preserved Ejection Fraction [Pubmed:27067668] |
| International journal of cardiology | Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. [pubmed:27448535] |
| Experimental and Therapeutic Medicine | Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure. [pubmed:28565777] |
| Journal of Cardiovascular Electrophysiology | Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. [pubmed:28127817] |
| Journal of Cardiovascular Ecography | Study of Vitamin D Status in Patients with Dilated Cardiomyopathy at a Teaching Hospital in North India. [pubmed:28465969] |
| International?Journal?of?Cardiology | 5-methoxytryptophan?is a?potential?marker?for?post-myocardial?infarction?heart?failure - apreliminary?approach?to?clinical?utility. [pubmed:27526355] |
| Cardiovasc Toxicol | Beneficial Effects of Ozone Therapy on Oxidative Stress, Cardiac Functions and Clinical Findings in Patients with Heart Failure Reduced Ejection Fraction [pubmed:28097518] |
| Sci Rep.? | Serial heart rhythm complexity changes in patients with anterior wall ST segment elevation myocardial infarction. [pubmed:28252107] |
| Journal of Cardiovascular Magnetic Resonance | The predictive capabilities of a novel cardiovascular magnetic resonance derived marker of cardiopulmonary reserve on established prognostic surrogate markers in patients with pulmonary vascular disease: results of a longitudinal pilot study [pubmed:28065166] |
| Repositório Institucional UNESP | AVALIA??O ELETROCARDIOGRáFICA AMBULATORIAL DE C?ES COM EHRLIQUIOSE MONOCíTICA CR?NICA [handle:11449] |
| Archivos de medicina veterinaria | Cardiac biomarkers in dogs with visceral leishmaniasis [S0301-732X2016000300004] |
| Original article | Evaluation of N-Terminal Pro Brain Natriuretic Peptide as a biomarker for clinical severity of heart failure in pediatric population [Attach432] |
| Evidence-Based Complementary and Alternative Medicine | Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathway [pubmed:28698735] |
| Scientific Reports | QSKL protects against myocardial apoptosis on heart failure via PI3K/Akt-p53 signaling pathway. [pubmed:29209026] |
| Vascular Pharmacology | Reversal effects of low-dose imatinib compared with sunitinib on monocrotaline-induced pulmonary and right ventricular remodeling in rats [pubmed:29100963] |
| Evidence-based complementary and alternative medicine | Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Rats [pubmed:28706558] |
| Europace | Prediction of electro-anatomical substrate using APPLE score and biomarkers [Pubmed:29893827] |
| Heart?Rhythm | Role of NT-proANP and NT-proBNP in patients with atrial fibrillation: Association with atrial fibrillation progression phenotypes [Pubmed:29604419] |
| American Journal of Hypertension | MicroRNAs in Peripheral Mononuclear Cells as Potential Biomarkers in Hypertensive Patients With Heart Failure With Preserved Ejection Fraction [Pubmed:29506053] |
| International Journal of Molecular?Medicine | Differential microRNA expression profiles and bioinformatics analysis between young and aging spontaneously hypertensive rats [Pubmed:29328372] |
| Biomarkers | Regulation of circulating chromogranin B levels in heart failure [Pubmed:29098879] |
| Lung | Effect of 6-min Walk Test on pro-BNP Levels in Patients with Pulmonary Arterial Hypertension [Pubmed:29564533] |
| International Journal of Clinical and Exp Medicine | Dynamic monitoring the level of NT-proBNP in assessing the value of cardiac function and prognosis in patients with systemic infection [ijcem0072283.pdf] |
| FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| International?Journal?of?Clinical?and?Experimental?Medicine | Qiliqiangxin ameliorates cardiac dysfunction in heart failure rats post myocardial infarction via activation of HIF/VEGF pathway [] |
| Pediatric?Hematology?Oncology?Journal | Decreasing cardiac iron overload with Amlodipine and Spirulina in children with β-thalassemia [Doi: 10.1016/j.phoj.2018.10.001] |
| Journal of?Clinical?Medicine | Acute Responses of Novel Cardiac Biomarkers to a 24-h Ultra-Marathon [Pubmed: 30625976] |
| Van Veterinary Journal | Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure. [] |
| Journal of Complementary and Integrative?Medicine | Biological markers for the effects of yoga as a complementary and alternative medicine [Pubmed: 30735481] |
| Frontiers in Pharmacology | Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway [Pubmed: 30890937] |
| Journal of Clinical Medicine | Potential Effects of CXCL9 and CCL20 on Cardiac Fibrosis in Patients with Myocardial Infarction and Isoproterenol-Treated Rats [Pubmed: 31083544] |
| PLoS One | Evaluation of heart rate variability and behavior of electrocardiographic parameters in dogs affected by chronic Monocytic Ehrlichiosis [Pubmed: 31125348] |
| Cardiovascular Research | Cardio-protective effect of the secretome of Sca-1+ and Sca-1-cells in heart failure–not equal, but equally important? Paracrine cell effects on heart failure [] |
| Advances in Medical Sciences | The potential role of inflammation in cryptogenic stroke [] |
| Disease Markers | Arterial Stiffness and Indices of Left Ventricular Diastolic Dysfunction in Patients with Embolic Stroke of Undetermined Etiology [Pubmed: 31612072] |
| Journal of Human Kinetics | Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy [Pubmed: 32148580] |
| Nature Communications | Preclinical development of a miR-132 inhibitor for heart failure treatment [Pubmed: 32005803] |
| MEDIATORS OF INFLAMMATION | Evaluating the Relationship of GDF-15 with Clinical Characteristics, Cardinal Features, and Survival in Multiple Myeloma [Pubmed: 33144847] |
| EUROPEAN HEART JOURNAL | CDR132L improves systolic and diastolic function in a large animal model of chronic heart failure [Pubmed: 33089304] |
| REPRODUCTIVE TOXICOLOGY | Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice [Pubmed: 33068716] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | Apela improves cardiac and renal function in mice with acute myocardial infarction [Pubmed: 32686917] |
| J Multidiscip Healthc | Left Ventricular Diastolic Dysfunction as Predictor of Unfavorable Prognosis After ESUS [33727824] |
| Oxid Med Cell Longev | κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a [34373768] |
| Biology | Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy [34356497] |
| Arch Acad Emerg Med | Risk Stratification of Pulmonary Thromboembolism using Brain Natriuretic Peptide and Troponin I; a Brief Report [Pubmed:35072097] |
| Transl Res | Interplay between mineral bone disorder and cardiac damage in acute kidney injury: from Ca2+ mishandling and preventive role of Klotho in mice to its potential?… [Pubmed:35077866] |
| Medicina (Kaunas) | The Key Role of Hepcidin-25 in Anemia in Multiple Myeloma Patients with Renal Impairment [Pubmed:35334593] |
| Acta Cardiol Sin | Prothymosin α Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion Injury [Pubmed:35273440] |
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