淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)
High Sensitive ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)
- 編號HEA946Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長3h
- 檢測范圍3.70-300pg/mL
- 靈敏度最小可檢測劑量小于等于1.24pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2911 ¥ 4158 ¥ 18711 ¥ 35343 ¥ 291060
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-104 | 93 |
| EDTA plasma(n=5) | 86-101 | 94 |
| heparin plasma(n=5) | 81-101 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-104% | 94-102% | 82-103% | 80-104% |
| EDTA plasma(n=5) | 79-95% | 90-101% | 90-105% | 81-104% |
| heparin plasma(n=5) | 79-92% | 79-93% | 89-101% | 95-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
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| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA946Hu12 | 淀粉樣蛋白β1-42(Ab1-42)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| SPA946Hu02 | 淀粉樣蛋白β1-42(Ab1-42)合成多肽 | Immunogen; Blocking Peptide. |
| CPA946Hu11 | 淀粉樣蛋白β1-42(Ab1-42)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA946Hu31 | 淀粉樣蛋白β1-42(Ab1-42)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA946Hu22 | 淀粉樣蛋白β1-42(Ab1-42)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA946Hu21 | 淀粉樣蛋白β1-42(Ab1-42)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA946Hu08 | 淀粉樣蛋白β1-42(Ab1-42)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA946Hu21 | 淀粉樣蛋白β1-42(Ab1-42)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA946Hu22 | 淀粉樣蛋白β1-42(Ab1-42)單克隆抗體 | WB; IHC; ICC; IP. |
| MEA946Hu | 淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA946Hu | 淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA946Hu | 淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA946Hu | 淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Neuropsychopharmacology | Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s Disease [Pubmed: 23493042] |
| Neuropharmacology and analgesia | The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1–42 seeding and short-term memory deficits in the Aβ25–35 mouse model of Alzheimer's disease [Pubmed: 23123349] |
| 世界科技研究與發(fā)展(World Sci-tech R & D) | 慢病毒介導(dǎo)的ＣＢＳ基因過表達(dá)對原代皮質(zhì)神經(jīng)元 [Globesci:Source] |
| 南方醫(yī)科大學(xué)學(xué)報(bào) | 外源性硫化氫對高糖條件下原代神經(jīng)元β淀粉樣蛋白的影響 [J-Smu:Source] |
| Neurochemical research | Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat Brain [Pubmed:24792732] |
| Indian J Biochem Biophys. | Amyloid ? lowering and cognition enhancing effects of ghrelin receptor analog [D-Lys (3)] GHRP-6 in rat model of obesity [Pubmed:25296496] |
| Cell Mol Neurobiol | Anesthetic Propofol Attenuates Apoptosis, Aβ Accumulation, and Inflammation Induced by Sevoflurane Through NF-κB Pathway in Human Neuroglioma Cells [PubMed: 25809614] |
| Neuropsychiatr Dis Treat | Autophagy dysfunction upregulates beta-amyloid peptides via enhancing the activity of γ-secretase complex [PubMed: 26316755] |
| Neurochem Int | Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS2 transgenic model of Alzheimer's disease [PubMed: 25882166] |
| Journal of Alzheimer’s Parkinsonism & Dementia | The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Rats [open-access:] |
| 世界科技研究與發(fā)展 | 芝麻素對高糖條件下PC12細(xì)胞Aβ和BACE-1表達(dá)的影響 [article:10299] |
| Am J Alzheimers Dis Other Demen. | Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease [Pubmed:26993489] |
| Св?т медицини та б?олог?? | ОСОБЕННОСТИ ПРОЦЕССОВ ФОРМИРОВАНИЯ ПОКАЗАТЕЛЕЙ ФУНКЦИОНАЛЬНОГО СОСТОЯНИЯ ВЫСШЕЙ НЕРВНОЙ ДЕЯТЕЛЬНОСТИ И ХАРАКТЕРИСТИКИ ВНИМАНИЯ СТУДЕНТОВ [SMB-2016-03-062.pdf] |
| 世界科技研究與發(fā)展 | 芝麻素對高糖條件下PC12 細(xì)胞A? 和BACE 1 表達(dá)的影響?yīng)?[QK:92621A] |
| Archives of Pharmacal Research | Geniposide attenuates the level of Aβ1–42?via enhancing leptin signaling in cellular and APP/PS1 transgenic mice [pubmed:28160136] |
| Food Funct. | Effects of dietary glucocerebrosides from sea cucumber on the brain sphingolipid profiles of mouse models of Alzheimer's disease. [pubmed:28232989] |
| Scientific Reports | Norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s disease [articles:srep41041.ris] |
| Journal?of?Medicinal?Food | Cerebrosides from Sea Cucumber Protect Against Oxidative Stress in SAMP8 Mice and PC12 Cells [pubmed:28406733] |
| Journal?of?Nutritional?Biochemistry | Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat diet [Pubmed:29986309] |
| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats [Pubmed:30028991] |
| Journal of Affective Disorders | Sub-chronic celecoxib prevents soluble beta amyloid-induced depressive-like behaviour in rats [Pubmed:29879605] |
| Biomedicine & Pharmacotherapy | Punicalagin ameliorates the elevation of plasma homocysteine, amyloid-β, TNF-α and apoptosis by advocating antioxidants and modulating apoptotic mediator proteins in brain modulating apoptotic mediator?… [Pubmed:29579708] |
| Journal of Agricultural and?Food?Chemistry | Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic Mice [Pubmed:29695154] |
| Neurotoxicology | Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats [Pubmed:29522778] |
| Biochemical and Biophysical?Research?Communications | CART peptide activates the Nrf2/HO-1 antioxidant pathway and protects hippocampal neurons in a rat model of Alzheimer's disease [Pubmed:29777699] |
| Oncotarget | Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse?… [Pubmed:29731975] |
| Experimental?and?Therapeutic?Medicine | Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β?amyloid production and tau hyperphosphorylation [Doi: 10.3892/etm.2018.6860] |
| 非編碼RNA miR-137 調(diào)控tau 蛋白磷酸化和L 型鈣離子通道CACNA1C 參與阿爾茨海默病的發(fā)生 [] | |
| 臺灣農(nóng)業(yè)研究 | (臺灣農(nóng)業(yè)研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏癥罹病風(fēng)險(xiǎn)初探 [] |
| The Efficacy of Sauropus androgynus Leaves Extract To Improve Cognitive Function in Wistar Rats Induced Alzheimer's [] | |
| Neurotoxicity Research | Effect of Aluminum-Maltolate on the Content of Aβ Protein and the Expression of ApoER2, VLDLRs, and LRP1 in PC12-ApoE4 Cells [Pubmed: 30649678] |
| Neurochemical?Research | Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS1 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic Inflammation [Pubmed: 30756214] |
| Molecular and Cellular Biochemistry | Repetitive transcranial magnetic stimulation protects mice against 6-OHDA-induced Parkinson's disease symptoms by regulating brain amyloid β level [] |
| PLoS One | Obstructive sleep apnea may increase the risk of Alzheimer's disease [Pubmed: 31487291] |
| Metabolic Brain Disease | Nanoformulated ellagic acid ameliorates pentylenetetrazol-induced experimental epileptic seizures by modulating oxidative stress, inflammatory cytokines and?… [Pubmed: 31728888] |
| 博士論文 | Neurobiological determinants of depressive-like symptoms in rodents [] |
| Pharmacognosy Magazine | Mahanimbine-induced neuroprotection via cholinergic system and attenuated amyloidogenesis as well as neuroinflammation in lipopolysaccharides-induced?… [] |
| biosciences biotechnology research asia | Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet. [] |
| Neurochem Res | Ex Vivo Investigation of Bexarotene and Nicotinamide Function as a Protect?ve Agent on Rat Synaptosomes Treated with A|? (1-42) [33428094] |
| Food Funct | Comparative study on the effects of phosphatidylserine rich in DHA and EPA on A|?-intervened Alzheimer's disease using cell models [33876786] |
| Int J Neurosci | Protective effects of repetitive transcranial magnetic stimulation against 6-OHDA-induced Parkinson's symptoms in a mice model: the key role of miR-409-3p/PDHB ?- [34078216] |
| Brain Res Bul | Immunization with A|?3-10-KLH vaccine improves cognitive function and ameliorates mitochondrial dysfunction and reduces Alzheimer's disease-like pathology in Tg ?- [34044034] |
| PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | The effect of a 6-month Ketogenic Medium-Chain Triglyceride Supplement on Plasma Cardiometabolic and Inflammatory Markers in Mild Cognitive??- [33906081] |
| Crystals | Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent) [] |
| Neurosci Lett | GPNMB mitigates Alzheimer's disease and enhances autophagy via suppressing the mTOR signal [34695452] |
| Arch Gerontol Geriatr | The influence of vinpocetine alone or in combination with Epigallocatechin-3-gallate, Coenzyme COQ10, Vitamin E and Selenium as a potential neuroprotective?… [34706318] |
| Metab Brain Dis | Probiotics ameliorates LPS induced neuroinflammation injury on Aβ 1–42, APP, γ-β secretase and BDNF levels in maternal gut microbiota and fetal?… [Pubmed:35312928] |
| Medicinal Chemistry Research | New Thienopyridine Scaffold as An Antidementia Lead: In Silico Study and Biological Screening [] |
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