孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)
Instant ELISA Kit for Progesterone (PG)
P4; Pregn-4-Ene-3,20-Dione
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-105 | 102 |
| EDTA plasma(n=5) | 83-90 | 87 |
| heparin plasma(n=5) | 79-101 | 80 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-96% | 91-99% | 78-97% | 94-103% |
| EDTA plasma(n=5) | 79-89% | 82-104% | 83-101% | 87-99% |
| heparin plasma(n=5) | 86-93% | 92-103% | 93-101% | 98-105% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C溫育30分鐘;
3. 甩干，加檢測溶液A100µL，37°C溫育30分鐘;
4. 洗板3次；
5. 加檢測溶液B100µL， 37°C孵育10分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10－20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)單克隆抗體包被于96孔微孔板中，制成固相載體，向微孔中加入標準品或標本以及同時加入HRP標記孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)的多克隆抗體，孵育結束徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的被檢測物濃度呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關產品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPA459Ge31 | 孕酮(PG)鑰孔血藍蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| CPA459Ge11 | 孕酮(PG)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| CPA459Ge21 | 孕酮(PG)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| CPA459Ge41 | 孕酮(PG)人血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAA459Ge01 | 孕酮(PG)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA459Ge02 | 孕酮(PG)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA459Ge71 | 孕酮(PG)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA459Ge24 | 孕酮(PG)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA459Ge21 | 孕酮(PG)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA459Ge23 | 孕酮(PG)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA459Ge22 | 孕酮(PG)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA459Ge | 孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA459Ge | 孕酮(PG)檢測試劑盒(酶聯免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA459Ge | 孕酮(PG)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA459Ge | 孕酮(PG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA459Ge11 | 孕酮(PG)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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