1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for 1,25-Dihydroxyvitamin D3 (DHVD3) ,etc. by FLIA (Flow Luminescence Immunoassay)
DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號LMA467Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長1.5h
- 檢測范圍1.95-2000pg/mL
- 靈敏度最小可檢測劑量小于等于0.65 pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 98 |
| EDTA plasma(n=5) | 78-95 | 83 |
| heparin plasma(n=5) | 87-95 | 91 |
| sodium citrate plasma(n=5) | 95-103 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 91-104% | 94-102% | 85-98% |
| EDTA plasma(n=5) | 94-102% | 87-94% | 94-102% | 87-95% |
| heparin plasma(n=5) | 80-94% | 88-95% | 99-105% | 84-104% |
| sodium citrate plasma(n=5) | 87-101% | 80-92% | 82-92% | 88-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CEA467Ge | 1,25-二羥基維生素D3(DHVD3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA467Ge | 1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Nephrol Dial Transplant. | Treatment of established left ventricular hypertrophy with fibroblast growth factor receptor blockade in an animal model of CKD [Pubmed:24875663] |
| Endocrinology. | Vitamin D Prevents Podocyte Injury via Regulation of Macrophage M1/M2 Phenotype in Diabetic Nephropathy Rats [Pubmed:25188527] |
| Tumor Biology | Calcitriol?induced?redox?imbalance and DNA breakage in cells sharing a common metabolic feature of malignancies: Interaction with cellular copper (II) ions leads to the production of reactive oxygen species. [Pubmed:25547436] |
| International Journal of Life Sciences Research | Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats [profile:303311214] |
| Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats [:] | |
| Apoptosis | Calcitriol–copper interaction leads to non enzymatic, reactive oxygen species mediated DNA breakage and modulation of cellular redox scavengers in hepatocellular … [article:10.1007] |
| ?in vivo | Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level [pubmed:28064234] |
| Hypertension Research | Glucocorticoid exposure induces preeclampsia via dampening 1,25-dihydroxyvitamin D3 [pubmed:29093562] |
| International?Journal?of?Clinical?and?Experimental?Medicine | Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats [] |
| Journal of Clinical and Translational Endocrinology | Dobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism [] |
| International Journal of Environmental Research and Public Health | Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats [Pubmed: 31739465] |
| Ecotoxicology and Environmental Safety | Sodium dehydroacetate induces cardiovascular toxicity associated with Ca2+ imbalance in zebrafish [] |
| EXPERIMENTAL AND MOLECULAR MEDICINE | Metabolic activities affect femur and lumbar vertebrae remodeling, and anti-resorptive risedronate disturbs femoral cortical bone remodeling [33436949] |
| Life Sci | SWIM domain protein ZSWIM4 is required for JAK2 inhibition resistance in breast cancer [34102191] |
| Nutrients | Effect of Dapagliflozin and Magnesium Supplementation on Renal Magnesium Handling and Magnesium Homeostasis in Metabolic Syndrome [34836340] |
