8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay)
8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG
（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）
- 編號(hào)LMA660Ge
- 物種Pan-species (General，通用) 相同的名稱(chēng)，不同的物種。
- 實(shí)驗(yàn)方法競(jìng)爭(zhēng)抑制
- 反應(yīng)時(shí)長(zhǎng)1.5h
- 檢測(cè)范圍5.86-6000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于1.953 pg/mL.
- 樣本類(lèi)型Serum, plasma and other biological fluids
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
- 價(jià)格 ¥ 2980 ¥ 3094 ¥ 3266 ¥ 3495 ¥ 3725 ¥ 4068 ¥ 4584 ¥ 5730 添加到價(jià)格計(jì)算器 計(jì)算器
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷(xiāo)商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 83-101 | 95 |
| EDTA plasma(n=5) | 78-98 | 89 |
| heparin plasma(n=5) | 87-101 | 91 |
| sodium citrate plasma(n=5) | 86-104 | 95 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-97% | 91-105% | 89-98% | 79-95% |
| EDTA plasma(n=5) | 92-105% | 81-91% | 87-95% | 82-97% |
| heparin plasma(n=5) | 86-101% | 79-99% | 84-95% | 91-98% |
| sodium citrate plasma(n=5) | 80-102% | 81-104% | 91-99% | 89-96% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測(cè)溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測(cè)溶液A50μL，
37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)和未標(biāo)記的8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競(jìng)爭(zhēng)結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CEA660Ge | 8-羥基脫氧鳥(niǎo)苷(8-OHdG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA660Ge | 8-羥基脫氧鳥(niǎo)苷(8-OHdG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA660Ge | 8-羥基脫氧鳥(niǎo)苷(8-OHdG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Yonsei Med J. | Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. [Pubmed: 24142649] |
| J Histochem Cytochem | Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats [PubMed: 26385569] |
| Diabetes/Metabolism Research and Reviews | Reduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of … [doi:10.1002] |
| Nutrition Journal | Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study [Pubmed:27071648] |
| Diabetology & Metabolic Syndrome | Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection [pmc:PMC4855822] |
| UVaDOC | Dieta hipercalórica e hipoxia intermitente crónica como modelos de obesidad y apnea obstructiva del sue?o. Significado de la leptina en el control respiratorio [Tesis1149-161108.pdf] |
| Journal of Diabetes Mellitus | The Activity of Serum 8-Iso-Prostaglandin F2α as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic Hyperglycemia [profile:Wesam_Nasef3] |
| Indian Journal of Hematology and Blood Transfusion | Oleuropein Ameliorates Cisplatin-induced Hematological Damages Via Restraining Oxidative Stress and DNA Injury [article:10.1007] |
| Journal?of?Diabetes?Research | Oxidative?Damage?to the?Salivary?Glands?of?Rats?with?Streptozotocin-Induced?Diabetes-TemporalStudy:?Oxidative?Stress?and?Diabetic?Salivary?Glands. [pubmed:27478848] |
| Front Physiol. | Oxidative Modification in the Salivary Glands of High Fat-Diet Induced Insulin Resistant Rats. [pubmed:28184199] |
| Archives of Oral Biology | Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats [pubmed:28926744] |
| BioMed Research International | Oxidative modification of biomolecules in the non-stimulated and stimulated saliva of patients with morbid obesity treated with bariatric surgery [10.1155:2017] |
| Oncotarget | Chemopreventive effects of atractylenolide II on mammary tumorigenesis via activating Nrf2-ARE pathway [pubmed:29100404] |
| International Journal of Molecular Sciences | Antioxidant Defence, Oxidative Stress and Oxidative Damage in Saliva, Plasma and Erythrocytes of Dementia Patients. Can Salivary AGE be a Marker of Dementia? [pubmed:29053628] |
| Antioxidants and Redox Signaling | Chemokine Receptor CXCR4 Plays a Crucial Role in Mediating Oxidative StressInduced Podocyte Injury [10.1089:ars.2016.6758] |
| Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats [ISSN:1940-5901/IJCEM0048822] | |
| Biological Trace Element Research | Protective Effect of Origanum Oil on Alterations of Some Trace Elements and Antioxidant Levels Induced by Mercuric Chloride in Male Rats [pubmed:28616770] |
| HAYATI Journal of Biosciences | Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult SpragueeDawley Rats [10.1016/j.hjb.2017.08.007] |
| Scientific Reports | Risk of glomerular filtration rate decline in patients with hypertrophic cardiomyopathy and obstructive sleep apnoea [pubmed:29234143] |
| Journal of Pain Research | Sex differences in complex regional pain syndrome type I (CRPS-I) in mice [pubmed:28831269] |
| HAYATI Journal of Biosciences | Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult Sprague–Dawley Rats [10.1016/j.hjb.2017.08.007] |
| Oxidative Medicine and Cellular Longevity | Effect of N-Acetylcysteine on Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in a Rat Model of Insulin Resistance [Pubmed:29636851] |
| Oxidative medicine and cellular longevity | Exposure to Ti4Al4V Titanium Alloy Leads to Redox Abnormalities, Oxidative Stress, and Oxidative Damage in Patients Treated for Mandible Fractures [Pubmed:30013720] |
| Journal of Cardiovascular Development and Disease | Oxidative DNA Damage and Carotid Intima Media Thickness as Predictors of Cardiovascular Disease in Prediabetic Subjects [Pubmed:29518984] |
| Reproductive?Biology | Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring [Pubmed:30005909] |
| Oxidative medicine and cellular longevity | Redox Balance, Antioxidant Defense, and Oxidative Damage in the Hypothalamus and Cerebral Cortex of Rats with High Fat Diet-Induced Insulin Resistance [] |
| Journal of Pharmacological Reports | Journal of Pharmacological Reports [Pubmed: 27489887] |
| Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Cross-sectional correlates of oxidative stress and inflammation with glucose intolerance in prediabetes [] |
| BioMed Research International | Oral Administration of Lactobacillus delbrueckii during the Suckling Phase Improves Antioxidant Activities and Immune Responses after the Weaning Event in a Piglet?… [] |
| Oxidative Medicine and Cellular Longevity | Luteolin Attenuates Cardiac Ischemia/Reperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function [] |
| Oxidative Medicine and Cellular Longevity | Dendrobium Officinale Polysaccharides Protect against MNNG-Induced PLGC in Rats via Activating the NRF2 and Antioxidant Enzymes HO-1 and NQO-1 [] |
| International Journal of Pharmaceutical Research & Allied Sciences | Prophylactic Impact of Beta vulgaris L in Ameliorating Cyclosporine A-Induced Hepatotoxicity in Rats. [] |
| Journal of Diabetes and Metabolism | Assessment of 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as a Diagnostic Marker for Diabetic Nephropathy in Type 1 Egyptian Diabetic Patients [] |
| Journal of Food Biochemistry | Dietary supplementation of barley and/or dates attenuate hypercholesterolemic‐induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic?… [Pubmed: 31373029] |
| environmental science and pollution research | Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains [Pubmed: 31468353] |
| Oxidative Medicine and Cellular Longevity | Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population [Pubmed: 32215181] |
| Journal of Clinical Medicine | Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents [Pubmed: 32079369] |
| Frontiers in Pharmacology | Hydrogen Sulfide Attenuates Particulate Matter-Induced Emphysema and Airway Inflammation Through Nrf2-Dependent Manner [Pubmed: 32116706] |
| Odontology | Salivary 8-hydroxyguanosine levels in smokers and non-smokers with chronic periodontitis [Pubmed: 32065311] |
| FREE RADICAL RESEARCH | Negative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide?… [Pubmed: 32985285] |
| JOURNAL OF FOOD SCIENCE | Lycopene protects neuroblastoma cells against oxidative damage via depression of ER stress [Pubmed: 32885410] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181] |
| Lipids Health Dis | Triglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism … [Pubmed: 32487177] |
| Theranostics | Rescuing Dicer expression in inflamed colon tissues alleviates colitis and prevents colitis-associated tumorigenesis [Pubmed: 32483416] |
| Journal of Clinical Medicine | Systemic Redox Imbalance in Patients with Chronic Granulomatous Disease [Pubmed: 32397350] |
| Oxidative Medicine and Cellular Longevity | NAC Supplementation of Hyperglycemic Rats Prevents the Development of Insulin Resistance and Improves Antioxidant Status but Only Alleviates General?… [Pubmed: 33274008] |
| OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross … [Pubmed: 32509152] |
| oxidative medine cellular longevity | Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β … [Pubmed: 32617139] |
| MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | Induction of genotoxicity and mutagenic potential of heavy metals in Thai occupational workers [Pubmed: 32928371] |
| Journal of Functional Foods | Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets [] |
| Journal of Diabetes Research | Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China [] |
| The potential protective role of gamma-irradiated vaccine versus Punica granatum treatment against murine trichinellosis [] | |
| Mutat Res Gen Tox En [] | |
| Electroacupuncture ameliorates motor dysfunction via inhibiting p66Shc-mediated oxidative stress and endoplasmic reticulum stress in rats with spinal cord … [] | |
| Journal of Skin and Stem Cell | 8-OHdG and hOGG1 as Oxidative DNA Damage Markers in Acne Vulgaris Patients Under Isotretinoin Treatment [] |
| Redox Biol | Biomarkers of nucleic acid oxidation¨Ca summary state-of-the-art [33579665] |
| Theranostics | Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage [33897894] |
| Exp Ther Med | Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats [34093766] |
| chemosphere | Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective [33662726] |
| Food Funct. | Sestrin2 is involved in Nrf2-regulated antioxidative signaling pathway in luteolin-induced prevention of the diabetic rat heart from ischemia/reperfusion injury [33900303] |
| Aquaculture Reports | Growth performance, antioxidant capacity, tissue fatty acid composition and lipid metabolism of juvenile green mud crab Scylla paramamosain in response to??- [] |
| Ann Behav Med | Role of Yoga and Its Plausible Mechanism in the Mitigation of DNA Damage in Type-2 Diabetes: A Randomized Clinical Trial [34180500] |
| Cancer Manag Res | Association of Tumour Microenvironment with Protein Glycooxidation, DNA Damage, and Nitrosative Stress in Colorectal Cancer [34408493] |
| The Journal of Clinical Endocrinology & Metabolism | High Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFα [34562085] |
| Ecotoxicol Environ Saf | Chromium induced neurotoxicity by altering metabolism in zebrafish larvae [34781135] |
| Toxins | Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy [34437430] |
| J Int Med Res | Body mass index is associated with Helicobacter pylori infection and increased oxidative DNA damage in an obese population [Pubmed:35209724] |
| Ann Transplant | Biochemical and Molecular Studies of Different Parameters as Markers for Nephropathy in Type 1 Diabetic Patients [Pubmed:34751188] |
| Oxidative Medicine and Cellular Longevity | Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway [Pubmed:35602095] |
留言咨詢(xún)
