淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Amyloid Beta Peptide 1-42 (Ab1-42) ,etc. by FLIA (Flow Luminescence Immunoassay)
(注:單次混測多因子不超過8個(gè)指標(biāo) )
- 編號LMA946Mi
- 物種Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長1.5h
- 檢測范圍0.29-300pg/mL
- 靈敏度最小可檢測劑量小于等于0.097 pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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特異性
本試劑盒對檢測淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法)具有較高的靈敏度。
經(jīng)檢測小鼠、大鼠之間有100%的交叉反映。
回收率
分別于定值血清及血漿樣本中加入一定量的淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標(biāo)樣品),重復(fù)測定并計(jì)算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 80-89 | 86 |
EDTA plasma(n=5) | 96-105 | 101 |
heparin plasma(n=5) | 79-90 | 78 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定,每個(gè)樣本使用同一試劑盒重復(fù)測定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-95% | 98-105% | 88-101% | 78-104% |
EDTA plasma(n=5) | 78-89% | 89-102% | 78-93% | 86-99% |
heparin plasma(n=5) | 85-99% | 93-102% | 90-98% | 90-99% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A)標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL,加檢測溶液A50μL,
37°C酶標(biāo)板振蕩器孵育60分鐘;
3. 磁吸洗板3次;
4. 加檢測溶液B100μL,37°C振動孵育30分鐘;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA,標(biāo)記的淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合,然后將未結(jié)合物洗凈后,加入PE標(biāo)記的親和素,再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
MAA946Mi21 | 淀粉樣蛋白β1-42(Ab1-42)單克隆抗體 | WB; IHC; ICC; IP. |
MAA946Mi22 | 淀粉樣蛋白β1-42(Ab1-42)單克隆抗體 | WB; IHC; ICC; IP. |
CEA946Mi | 淀粉樣蛋白β1-42(Ab1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA946Mi | 淀粉樣蛋白β1-42(Ab1-42)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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