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腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay)

GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

（注：單次混測多因子不超過8個指標 ）

  • 腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 93-102 97
EDTA plasma(n=5) 80-101 79
heparin plasma(n=5) 87-97 93

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 89-102% 78-101% 78-99% 98-105%
EDTA plasma(n=5) 97-104% 92-105% 89-96% 94-101%
heparin plasma(n=5) 90-104% 78-99% 97-104% 82-92%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的腦鈉素(BNP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應用(僅供研究使用，不用于臨床診斷！)
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參考文獻

雜志 參考文獻
Heart and Circulatory Physiology B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source]
PLoS One Renalase deficiency in heart failure model of rats_a potential mechanism underlying circulating norepinephrine accumulation. [PubMed: 21297953]
International Journal of Toxicology Cardiac Troponin T and NT-proBNP as Biomarkers of Early Myocardial Damage in Amitriptyline-Induced Cardiovascular Toxicity in Rats [Pubmed: 24055824]
JACC ELEVATED ST2 SERUM LEVELS A BIOMARKER FOR CARDIOMYOPATHY IN DUCHENNE MUSCULAR DYSTROPHY [Onlinejacc:Source]
J Urol.? Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing? [Pubmed:24679871]
Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca2 [PubMed: 26706290]
Vlaams Diergeneeskundig Tijdschrift Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study [Publication: 5950761]
Physiological Research Renal Sympathetic Denervation Improves Cardiac Dysfunction [PubMed: 25804096]
Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro [Pubmed:26706290]
J Ethnopharmacol Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathway [Pubmed:26970569]
Echocardiography. Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction. [pubmed:27779336]
SpringerPlus The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … [pubmed:27652141]
Heart Lung Circ. Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. [pubmed:27555054]
Clinica?Chimica?Acta? Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathy [pubmed:27693530]
Scientific Reports Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP. [pubmed:28091624]
Molecular Medicine Report Intermedin improves cardiac function and sympathetic neural remodeling in a rat model of post myocardial infarction heart failure [pubmed:28627670]
PLoS One Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788]
Acta Physiologica Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload [pubmed:27575955]
International Immunopharmacology Metformin attenuated endotoxin-induced acute myocarditis via activating AMPK [pubmed:28410530]
Drug Delivery Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy. [pubmed:29063791]
Science?of the?Total?Environment Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and [Pubmed:29727991]
Scientific Reports An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease [Pubmed:29895976]
International Journal of Cardiology Cardioprotective effect of substance P in a porcine model of acute myocardial infarction [Pubmed:29884290]
International?Journal?of?Cardiology High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failure [Pubmed:30072155]
Evidence-based?Complementary and Alternative?Medicine? Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313]
Molecular?Medicine?Reports? Hydrogen?rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781]
Pediatric Cardiology Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease [Pubmed:29392348]
BMC?Pharmacology and Toxicology Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis [Pubmed:29321061]
中國病理生理雜志 和肽素水平對大鼠心腎綜合征的早期預測價值 [filename:ZBLS201802013]
FASEB?Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922]
Toxicology Mechanisms and?Methods Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats [Pubmed: 30426860]
Van Veterinary Journal Van Veterinary Journal []
Kidney?International Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217]
Journal?of?Cellular?Physiology Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes [Pubmed: 30982981]
Cardiology Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure [Pubmed: 31466059]
BIOMEDICINE & PHARMACOTHERAPY Pharmacodynamic and pharmacokinetic effects of S086, a novel angiotensin receptor neprilysin inhibitor [Pubmed: 32570118]
biochimica et biophysica acta-molecular basis of disease Empagliflozin improves left ventricular diastolic function of db/db mice [Pubmed: 32353614]
Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF|?1/Smad ?- [34116674]
Radiation-induced heart energy metabolism dysfunction leads to heart fibrosis []
Oxid Med Cell Longev κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a [34373768]
Mol Med Rep Radiation?induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues [34633055]
viruses Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model [34452454]
Peptides Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress [Pubmed:35218795]
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