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D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for D-Dimer (D2D) ,etc. by FLIA (Flow Luminescence Immunoassay)

D 2 Dimer

（注：單次混測多因子不超過8個指標 ）

  • D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 94-102 98
EDTA plasma(n=5) 89-97 93
heparin plasma(n=5) 98-105 101
sodium citrate plasma(n=5) 84-92 88

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 97-104% 88-99% 79-90%
EDTA plasma(n=5) 92-105% 90-98% 88-101% 93-102%
heparin plasma(n=5) 81-103% 83-97% 79-98% 96-105%
sodium citrate plasma(n=5) 78-104% 83-90% 92-99% 88-97%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
    37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。

贈品

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編號 適用物種：Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
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參考文獻

雜志 參考文獻
22 Determinants of the thrombogenic potential of multiwalled carbon nanotubes [ScienceDirect: S0142961211004819]
Journal of Thrombosis and Haemostasis Exogenous Activated Protein C Inhibits the Progression of Diabetic Nephropathy [Pubmed: 22236035]
PLOS ONE Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice [PlosOne: Source]
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India [Pubmed: 23992124]
Journal of Trauma and Acute Care Surgery Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine model [Lww:Source]
Journal of Thrombosis and Haemostasis Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells [Pubmed:25399514]
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261]
Medical Science Monitor Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic Coagulopathy [Pubmed:25676919]
Blood Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation. [PubMed: 25575539]
Am J Physiol Regul Integr Comp Physiol Procoagulant and Fibrinolytic Activity after Polytrauma in Rat [PubMed: 26632604]
hypertension Research Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat model [PubMed: 26155749]
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502]
JCI Insight. KLF2 and KLF4 control endothelial identity and vascular integrity. [pubmed:28239661]
The American Society of Anesthesiologists Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathy [pubmed:27841821]
32 Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target [pubmed:28289017]
The American Journal of Pathology Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulin [pubmed:28739343]
Atherosclerosis Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin. [pubmed:28923781]
28 Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Mice [pubmed:29162596]
Journal of Thrombosis and Thrombolysis Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo. [pubmed:28417266]
Journal?of?Investigative?Surgery Experimental Evaluation of a New Tissue Factor-Based Topical Hemostat (TT-173) for Treatment of Hepatic Bleeding [Pubmed: 30380352]
Marine Drugs The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230]
Intensive Care Med Exp Complement factor D is linked to platelet activation in human and rodent sepsis [34396466]
Thromb Res NLRP3 Inflammasome contributes to endotoxin-induced coagulation [Pubmed:35421682]
Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715]
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