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Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Procollagen III N-Terminal Propeptide (PIIINP) ,etc. by FLIA (Flow Luminescence Immunoassay)

P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide

（注：單次混測多因子不超過8個指標 ）

  • Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 83-97 88
EDTA plasma(n=5) 96-104 101
heparin plasma(n=5) 89-103 97
sodium citrate plasma(n=5) 78-101 82

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 80-90% 84-91% 89-101%
EDTA plasma(n=5) 98-105% 80-94% 78-101% 92-101%
heparin plasma(n=5) 88-102% 96-105% 80-93% 96-103%
sodium citrate plasma(n=5) 94-101% 96-104% 78-99% 78-101%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。

實驗原理

將Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

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