Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay)
(注:單次混測多因子不超過8個指標 )
- 編號LMB892Ra
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長1.5h
- 檢測范圍0.2-200pg/mL
- 靈敏度最小可檢測劑量小于等于0.067 pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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特異性
本試劑盒用于檢測Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 92-105 | 101 |
EDTA plasma(n=5) | 98-105 | 101 |
heparin plasma(n=5) | 92-101 | 97 |
sodium citrate plasma(n=5) | 81-92 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 93-105% | 80-95% | 94-105% | 86-97% |
EDTA plasma(n=5) | 87-101% | 87-104% | 95-102% | 79-94% |
heparin plasma(n=5) | 88-102% | 93-105% | 88-95% | 95-102% |
sodium citrate plasma(n=5) | 95-102% | 80-102% | 88-102% | 81-105% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠、檢測溶液A)標準品或樣本50μL及磁珠10μL,加檢測溶液A50μL,
37°C酶標板振蕩器孵育60分鐘;
3. 磁吸洗板3次;
4. 加檢測溶液B100μL,37°C振動孵育30分鐘;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠、標記VEGFA,標記的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合,然后將未結(jié)合物洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
CEB892Ra | Salusin肽α(SALa)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB892Ra | Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB892Ra11 | Salusin肽α(SALa)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Peptides | The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass [PubMed: 22884920] |
Department of Medical Biochemistry and Clinical Biochemistry | Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533] |
European Journal of Obstetrics & Gynecology and Reproductive Biology | Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. [PubMed: 23178004] |
Biotechnic & Histochemistry | Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome [Pubmed: 23957705] |
Peptides | Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus [ScienceDirect: S0196978113003100] |
Med Sci Monit. | Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome [Pubmed:Pmc4136931] |
Pediatr Nephrol. | Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? [Pubmed:25245503] |
European Journal of Dermatology | Serum salusin-α and salusin-β levels in patients with psoriasis [PubMed: 26055619] |
Gynecol Endocrinol | Serum salusins levels are increased and correlated positively with cyst size in ovarian endometrioma [PubMed: 26008602] |
Multiple Sclerosis and?Related?Disorders | Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis [Pubmed: 30738876] |
cardiology in the young | Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity [Pubmed: 31486354] |
Drug and Chemical Toxicology | Salusin-α mitigates diabetic nephropathy via inhibition of the Akt/mTORC1/p70S6K signaling pathway in diabetic rats [Pubmed: 31665937] |
Journal of international medical research | Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis [Pubmed: 32054363] |
J Ultrasound Med | Targeted Delivery of Salusin?\|á into Rabbit Carotid Arterial Endothelium Using SonoVue [33818784] |
Food Science & Nutrition | Inhibition of oxidative stress and apoptosis by camel milk mitigates cyclosporine?\induced nephrotoxicity: Targeting Nrf2/HO?\1 and AKT/eNOS/NO pathways [34136182] |