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Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay)

(注:單次混測多因子不超過8個指標 )

  • Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 92-105 101
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 92-101 97
sodium citrate plasma(n=5) 81-92 89

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 93-105% 80-95% 94-105% 86-97%
EDTA plasma(n=5) 87-101% 87-104% 95-102% 79-94%
heparin plasma(n=5) 88-102% 93-105% 88-95% 95-102%
sodium citrate plasma(n=5) 95-102% 80-102% 88-102% 81-105%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠、檢測溶液A)標準品或樣本50μL及磁珠10μL,加檢測溶液A50μL,
    37°C酶標板振蕩器孵育60分鐘;
3. 磁吸洗板3次;
4. 加檢測溶液B100μL,37°C振動孵育30分鐘;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠、標記VEGFA,標記的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合,然后將未結(jié)合物洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的Salusin肽α(SALa)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。

贈品

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編號 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
CEB892Ra Salusin肽α(SALa)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
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參考文獻

雜志 參考文獻
Peptides The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass [PubMed: 22884920]
Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533]
European Journal of Obstetrics & Gynecology and Reproductive Biology Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. [PubMed: 23178004]
Biotechnic & Histochemistry Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome [Pubmed: 23957705]
Peptides Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus [ScienceDirect: S0196978113003100]
Med Sci Monit. Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome [Pubmed:Pmc4136931]
Pediatr Nephrol. Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? [Pubmed:25245503]
European Journal of Dermatology Serum salusin-α and salusin-β levels in patients with psoriasis [PubMed: 26055619]
Gynecol Endocrinol Serum salusins levels are increased and correlated positively with cyst size in ovarian endometrioma [PubMed: 26008602]
Multiple Sclerosis and?Related?Disorders Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis [Pubmed: 30738876]
cardiology in the young Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity [Pubmed: 31486354]
Drug and Chemical Toxicology Salusin-α mitigates diabetic nephropathy via inhibition of the Akt/mTORC1/p70S6K signaling pathway in diabetic rats [Pubmed: 31665937]
Journal of international medical research Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis [Pubmed: 32054363]
J Ultrasound Med Targeted Delivery of Salusin?\|á into Rabbit Carotid Arterial Endothelium Using SonoVue [33818784]
Food Science & Nutrition Inhibition of oxidative stress and apoptosis by camel milk mitigates cyclosporine?\induced nephrotoxicity: Targeting Nrf2/HO?\1 and AKT/eNOS/NO pathways [34136182]
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