補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)
Wide-range ELISA Kit for Complement Component 3a (C3a)
- 編號WEA387Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.064ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2516 ¥ 3594 ¥ 16173 ¥ 30549 ¥ 251580
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 81-103 | 87 |
| heparin plasma(n=5) | 93-104 | 99 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-95% | 98-105% | 95-104% | 78-94% |
| EDTA plasma(n=5) | 97-105% | 94-103% | 92-105% | 80-97% |
| heparin plasma(n=5) | 83-97% | 83-101% | 90-103% | 79-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)與連接于固相載體上的抗體結合，然后加入生物素化的補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA387Hu01 | 補體成分3a(C3a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA387Hu01 | 補體成分3a(C3a)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA387Hu01 | 補體成分3a(C3a)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu27 | 補體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu21 | 補體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu22 | 補體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu23 | 補體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu24 | 補體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu29 | 補體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA387Hu25 | 補體成分3a(C3a)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA387Hu | 補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA387Hu | 補體成分3a(C3a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA387Hu | 補體成分3a(C3a)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA387Hu | 補體成分3a(C3a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA387Hu01 | 補體成分3a(C3a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Plos One | Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in Mice [Pubmed:26872035] |
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| Artificial?Cells?Nanomedicine and Biotechnology Journal | Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly?… [Pubmed:29873524] |
| Cytotherapy | Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation [Doi: 10.1016/j.jcyt.2018.07.007] |
| Journal of?NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis [Pubmed: 30850976] |
| Clinical Immunology | Pathogenic roles of anti-C1q antibodies in recurrent pregnancy loss [] |
| Materials Science and Engineering: C | Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys [] |
| Nanomedicine | Complement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion [33549818] |
| EXPERIMENTAL EYE RESEARCH | Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression [33493474] |
| FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease [Pubmed:35503088] |
