甲胎蛋白(AFP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Alpha-Fetoprotein (AFP)
aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
- 編號KSA153Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍3.12-200ng/mL
- 可適靈敏度1.17ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 5378 ¥ 8964 ¥ 16135 ¥ 31374 ¥ 53784
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產品包裝(模擬)
-
產品包裝(模擬)
-
產品圖片
-
通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對甲胎蛋白(aFP)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA153Hu02 | 甲胎蛋白(AFP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA153Hu01 | 甲胎蛋白(AFP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA153Hu01 | 甲胎蛋白(AFP)多克隆抗體 | WB; IHC |
| PAA153Hu02 | 甲胎蛋白(AFP)多克隆抗體 | WB |
| LAA153Hu71 | 甲胎蛋白(AFP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA153Hu81 | 甲胎蛋白(AFP)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA153Hu21 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA153Hu22 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA153Hu26 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA153Hu23 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA153Hu24 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA153Hu25 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA153Hu27 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA153Hu29 | 甲胎蛋白(AFP)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA153Hu02 | 抗甲胎蛋白(AFP)單克隆抗體 | FCM |
| FAA153Hu04 | 抗甲胎蛋白(AFP)單克隆抗體 | FCM |
| FAA153Hu06 | 抗甲胎蛋白(AFP)單克隆抗體 | FCM |
| FAA153Hu08 | 抗甲胎蛋白(AFP)單克隆抗體 | FCM |
| SEA153Hu | 甲胎蛋白(AFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA153Hu | 甲胎蛋白(AFP)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA153Hu | 甲胎蛋白(AFP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA153Hu01 | 甲胎蛋白(AFP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| BioFactors | Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model [PubMed: 20872761] |
| Food and Nutrition Sciences | Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. [Scirp: 5684] |
| Diabetes Research and Clinical Practice | Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats [ScienceDirect: S0168822711000520] |
| Life Sciences | Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver [ScienceDirect: S0024320511002700] |
| Annals of Hepatology | Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. [Hepatology: source] |
| Biomedicine & Aging Pathology | Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats [ScienceDirect: S2210522013000348] |
| European journal of pharmacology | Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme [Pubmed: 24530413] |
| Asian Pac J Cancer Prev. | Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. [Pubmed:25684529] |
| Journal of Analytical Atomic Spectrometry | Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry [Rsc:Source] |
| PLOS ONE | Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma [Pubmed:24927126] |
| BMC Med Imaging | Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma [PubMed: 25981587] |
| Tumour Biol | Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma [PubMed: 26643893] |
| Acta Veterinaria Brno | The concentrations of selected blood serum proteins in calves during the first three months of life [85:33] |
| Hepatobiliary Pancreat Dis Int | Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern [abstract:abstract4525.shtml] |
| Journal of Applied Pharmaceutical Science | Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats [2071_pdf.pdf] |
| Frontiers in Physiology? | Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats [v04n07a07a] |
| International Journal of?Clinical?and Experimental?Medicine | Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model [1345-1349] |
| Tumor Biology | Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway [pubmed:28651490] |
| Advances in Clinical and Experimental Medicine | Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles [ISSN 2451-2680] |
| Tumor biology | Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats [pubmed:28720064] |
| EBioMedicine | Lnc-PCDH9-13: 1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular Carcinoma [Pubmed:30045829] |
| Sensors and Actuators B: Chemical | One-pot synthesis of a highly selective carboxyl-functionalized superparamagnetic probes for detection of alpha-fetoprotein [10.1016:j.snb.2018.03.038] |
| Biomedicine & Pharmacotherapy | Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and [Pubmed:29669302] |
| Journal of Investigative Surgery | Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation [Pubmed:28107094] |
| Asian?Pacific?Journal of?Cancer?Prevention | Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats [Pubmed:29693337] |
| Antioxid Redox Signal | The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma [Pubmed:29687726] |
| Cancer Biology | Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer [] |
| Cancer Biology | Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine [] |
| Biomedicine & Pharmacotherapy | The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB [Pubmed: 30784932] |
| Asian Journal of Pharmacy and Pharmacology | Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma [] |
| American Journal of Transplantation | Circulating exosomal miR‐92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence [Pubmed: 31162867] |
| Environ Toxicol Pharmacol. | Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats [Pubmed: 31421311] |
| Hepatology Research | Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition [Pubmed: 31652385] |
| REDOX REPORT | Selective cytotoxic activity and protective effects of sodium ascorbate against hepatocellular carcinoma through its effect on oxidative stress and apoptosis in vivo and … [Pubmed: 32172678] |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440] |
| Nutr Cancer | Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation [Pubmed: 32718197] |
| Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles [] | |
| Microchemical Journal | Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS [] |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats [] | |
| Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo [] | |
| DEVELOPMENT | Blocking estrogen-induced AMH expression is crucial for normal follicle formation [33658225] |
| Cytokine | QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model [34564023] |
| Journal of Gastrointestinal Cancer | In Vivo Study of a Newly Synthesized Chromen-4-One Derivative as an Antitumor Agent Against HCC [34698995] |
留言咨詢
