雙調(diào)蛋白(AREG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Amphiregulin (AREG)
AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor
- 編號KSA006Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍15.6-1,000pg/mL
- 可適靈敏度6.5pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6048 ¥ 10080 ¥ 18144 ¥ 35280 ¥ 60480
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特異性
試劑盒中的抗體對雙調(diào)蛋白(AREG)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APA006Hu01 | 雙調(diào)蛋白(AREG)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA006Hu01 | 雙調(diào)蛋白(AREG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA006Hu01 | 雙調(diào)蛋白(AREG)多克隆抗體 | WB; IHC; ICC; IP. |
MAA006Hu23 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC; ICC; IP. |
MAA006Hu25 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC; ICC; IP. |
MAA006Hu22 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC |
MAA006Hu24 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC; ICC/IF |
MAA006Hu26 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC |
MAA006Hu21 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC; ICC; IP. |
MAA006Hu27 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC; ICC; IP. |
MAA006Hu29 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC; ICC; IP. |
LAA006Hu76 | 雙調(diào)蛋白(AREG)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEA006Hu | 雙調(diào)蛋白(AREG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA006Hu | 雙調(diào)蛋白(AREG)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA006Hu | 雙調(diào)蛋白(AREG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA006Hu01 | 雙調(diào)蛋白(AREG)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA006Hu01 | 雙調(diào)蛋白(AREG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Onkologie | Amphiregulin and Epiregulin Expression in Colorectal Carcinoma and the Correlation with Clinicopathological Characteristics [Karger: 315380] |
Br J Cancer. | Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer. [Pubmed:24800946] |
Cancer Sci | Association between serum ligands and the skin toxicity of anti-epidermal growth factor receptor antibody in metastatic colorectal cancer [PubMed: 25707609] |
J Cancer Res Clin Oncol | Amphiregulin confers trastuzumab resistance via AKT and ERK activation in HER2-positive breast cancer [PubMed: 26195282] |
Oncotarget.? | Serum?level?of?hepatocyte?growth?factor?is a?novel?marker?of?predicting?theoutcome?and?resistance?to the?treatment?with?trastuzumab?in HER2-positive patients with metastatic gastric cancer [Pubmed:26716644] |
Acta?Pharmacologica Sinica | Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi') [Pubmed: 19363516] |
International Journal of Biological Sciences | CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17 [] |
Neoplasia | Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364] |
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets [Pubmed: 33164313] |
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