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膜聯(lián)蛋白A1(ANXA1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Annexin A1 (ANXA1)

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

  • 膜聯(lián)蛋白A1(ANXA1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 膜聯(lián)蛋白A1(ANXA1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對膜聯(lián)蛋白A1(ANXA1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Plos one Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease [PubMed: PMC3794972]
African Journals Online Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2 [Ajol: Source]
Antimicrobial Agents and Chemotherapy Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic Response [Asm: Source]
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) [PubMed: 26718731]
Diabetes Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet Function [PubMed: 26470781]
PLoS One Annexin A1 Is Increased in the Plasma of Preeclamptic Women [PubMed: 26398190]
中國現(xiàn)代醫(yī)學(xué)雜志 China Journal of Modern Medicine 膜聯(lián)蛋白A1 和CA19-9 在膽管癌中的表達(dá)及其臨床意義 [article:8686]
arteriosclerosis thrombosis and vascular biology Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. [pubmed:28062503]
Clinical Science Mesenchymal stromal cells improve human islet function through released products and extracellular matrix. [pubmed:29101297]
Biomedicine & Pharmacotherapy Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity [Pubmed:29677533]
?International Journal of?Chronic?Obstructive Pulmonary Disease Annexin A1 is elevated in patients with COPD and affects lung fibroblast function [Pubmed:29440885]
Medical?Mycology Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients [Pubmed:29370415]
DEVELOPMENTAL NEUROPSYCHOLOGY EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT?… [pdf:3395]
Multiple sclerosis and related disorders Resolution of inflammation in neuromyelitis optica spectrum disorders [Pubmed: 30300851]
Acta Pharmaceutica Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines []
npj Science of Food Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites [Pubmed: 32133417]
FASEB JOURNAL ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway [Pubmed: 32908567]
Rev Soc Bras Med Trop Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients [33263684]
science advances Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK|á stability via selective autophagy [33523920]
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