胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Caspase 8 (CASP8)
CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
- 編號KSA853Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.117ng/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 6221 ¥ 10368 ¥ 18662 ¥ 36288 ¥ 62208
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特異性
試劑盒中的抗體對胱天蛋白酶8(CASP8)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA853Mu01 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA853Mu02 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA853Mu01 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; IHC; ICC; IP. |
PAA853Mu02 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; IHC; ICC; IP. |
LAA853Mu81 | 胱天蛋白酶8(CASP8)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAA853Mu71 | 胱天蛋白酶8(CASP8)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEA853Mu | 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA853Mu | 胱天蛋白酶8(CASP8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA853Mu01 | 胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Molecular Neurobiology | Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462] |
Journal of Ethnopharmacology | Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells [Pubmed:29555410] |
Clinical?And Experimental Pharmacology And Physiology? | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336] |
Environmental Toxicology | Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice [Pubmed: 31087429] |
Environ Toxicol Pharmacol. | Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats [Pubmed: 31421311] |
Journal of Biochemical and Molecular Toxicology | Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation [Pubmed: 32045101] |
life sciences | Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats [Pubmed: 32259601] |
Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
Int Immunopharmacol | Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling [Pubmed: 33199237] |
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways [Pubmed: 32942000] |
Environ Sci Pollut Res Int | Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?… [Pubmed: 32915453] |
J Biochem Mol Toxicol | Tranilast abrogates cisplatin?\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation [34047436] |
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