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高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Golgi Phosphoprotein 2 (GOLPH2)

GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73

  • 高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • 高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對高爾基蛋白73(GP73)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPB668Mu02 高爾基磷酸化蛋白2(GOLPH2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB668Mu01 高爾基磷酸化蛋白2(GOLPH2)活性蛋白 Cell?culture;?Activity?Assays.
RPB668Mu01 高爾基磷酸化蛋白2(GOLPH2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB668Mu02 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體 WB; IHC; ICC; IP.
PAB668Mu01 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體 WB; IHC; ICC; IP; FCM
LAB668Mu81 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAB668Mu71 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體(生物素標記) WB; IHC; ICC.
SEB668Mu 高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB668Mu 高爾基磷酸化蛋白2(GOLPH2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB668Mu01 高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Molecular & Cellular Proteomics LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues [Mcponline: 023507]
Afro-Egypt J Infect Endem Dis Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma [Ajied: Ajied_System_Files]
Journal of Applied Pharmaceutical Science Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients. [handle:123456789]
Oncology Letters Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinoma [pubmed:29113278]
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440]
Hepatobiliary & Pancreatic Diseases International Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation [Pubmed: 32171652]
Biomolecules Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-|êB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11??- [33540642]
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C []
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