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基質(zhì)Gla蛋白(MGP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Matrix Gla Protein (MGP)

MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

  • 基質(zhì)Gla蛋白(MGP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 基質(zhì)Gla蛋白(MGP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對基質(zhì)Gla蛋白(MGP)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Nephrology Dialysis Transplantation Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells [PubMed: PMC3611891]
Maturitas. Bone metabolism regulators and arterial stiffness in postmenopausal women [Pubmed: 23916080]
The Egyptian Rheumatologist Osteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity [ScienceDirect: S1110116414000076]
Toxicology Reports Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice [Researchgate:Source]
Lipids in Health and Disease Markers of increased cardiovascular risk in patients with chronic kidney disease [Biomedcentral:Source]
Human & Veterinary Medicine The behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins [Hvm:Source]
Pol Arch Med Wewn Effect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5 [PubMed: 26176325]
Lipids Health Dis. Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study [pmc:PMC4739105]
Kidney Blood Press Res. Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease [Pubmed:27100101]
Archives of?Oral?Biology Matrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus [pubmed:27348051]
Cell?Death?&?Disease Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis [Pubmed:29880866]
Urology High concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein [Pubmed:29935264]
Clinica?Chimica?Acta Serum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases [Pubmed: 30597137]
Biological?Trace?Element?Research Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients [Pubmed: 30847765]
Renal Failure Relationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients [Pubmed: 31538831]
Heliyon Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4 [Pubmed: 32322728]
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