腎足蛋白(NPHN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Nephrin (NPHN)
NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor
- 編號KSA937Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.124ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特異性
試劑盒中的抗體對腎足蛋白(NPHN)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA937Ra01 | 腎足蛋白(NPHN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA937Ra01 | 腎足蛋白(NPHN)多克隆抗體 | WB; IHC; ICC; IP. |
MAA937Ra21 | 腎足蛋白(NPHN)單克隆抗體 | WB; IHC; ICC; IP. |
SEA937Ra | 腎足蛋白(NPHN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA937Ra | 腎足蛋白(NPHN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA937Ra01 | 腎足蛋白(NPHN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Yonsei medical journal | The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia. [pubmed:28120572] |
Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788] |
British Journal of Haematology? | Renal protection by atorvastatin in a murine model of sickle cell nephropathy [Pubmed:29527679] |
Journal of Clinical Medicine | Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney Disease [Pubmed: 32961903] |
Disease Markers | Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study [34707724] |
Biomed Rep | Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugs [Pubmed:35620307] |
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