神經(jīng)生長因子(NGF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
- 編號KSA105Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍15.6-1,000pg/mL
- 可適靈敏度6.2pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6048 ¥ 10080 ¥ 18144 ¥ 35280 ¥ 60480
- 欲了解實際交易價格和更多情況,請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
特異性
試劑盒中的抗體對神經(jīng)生長因子(NGF)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA105Hu02 | 神經(jīng)生長因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA105Hu61 | 神經(jīng)生長因子(NGF)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA105Hu01 | 神經(jīng)生長因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA105Hu01 | 神經(jīng)生長因子(NGF)活性蛋白 | Cell?culture;?Activity?Assays. |
EPA105Hu61 | 神經(jīng)生長因子(NGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA105Hu02 | 神經(jīng)生長因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
PAA105Hu01 | 神經(jīng)生長因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
PAA105Hu06 | 神經(jīng)生長因子(NGF)多克隆抗體 | WB; IHC; ICC; IP. |
LAA105Hu71 | 神經(jīng)生長因子(NGF)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA105Hu25 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA105Hu27 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA105Hu29 | 神經(jīng)生長因子(NGF)單克隆抗體 | IHC |
MAA105Hu21 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB |
MAA105Hu22 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB,ICC/IF |
MAA105Hu24 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA105Hu23 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB |
FAA105Hu02 | 抗神經(jīng)生長因子(NGF)單克隆抗體 | Flow cytometry. |
SEA105Hu | 神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA105Hu | 神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA105Hu | 神經(jīng)生長因子(NGF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA105Hu | 神經(jīng)生長因子(NGF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA105Hu01 | 神經(jīng)生長因子(NGF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
The Tohoku Journal of Experimental Medicine | Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats [Pubmed:25212248] |
International Journal of Pharmaceutics | Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs [Pubmed:25445537] |
Yonsei Medical Journal | NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766] |
Molecular and Cellular Endocrinology | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971] |
Mol Cell Endocrinol | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971] |
Neurosciences (Riyadh) | Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. [PubMed: 25864063] |
Neurotoxicity Research | Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures [Pubmed:27189478] |
Psychoneuroendocrinology | Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism [Pubmed:26771945] |
Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
The Journal of Maternal-Fetal & Neonatal Medicine | Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639] |
IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
biblioteca.uva | Evaluación de la secreción de factores neurotróficos por las células MSV en cocultivo con neurorretinas porcinas, en función de la dosis celular y del tiempo de cultivo [10324/27440] |
Progress in Neuro-Psychopharmacology & Biological Psychiatry | Antidepressant drugs for beta amyloid-induced depression: A new standpoint? [pubmed:28499898] |
Osteoarthritis and Cartilage | Mechanical loading of intervertebral disc modulates microglia proliferation, activation, and chemotaxis [Pubmed:29723636] |
Stem?Cell?Research?&?Therapy | Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model [Pubmed:29335016] |
Pharmacological?Research? | N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype [Pubmed:29203442] |
Experimental Neurology | Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia [Pubmed:30012511] |
Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats [Pubmed:30028991] |
Experimental Dermatology | 3‐‐Laurylglyceryl ascorbate improves the development of sensitive skin through the reduction of oxidative stress [Pubmed: 30698879] |
BioMed Research International | Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling [] |
International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807] |
Experimental and Therapeutic Medicine | Involvement of neurotrophic signaling in doxorubicin?induced cardiotoxicity [Pubmed: 32010279] |
Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy [Pubmed: 32165929] |
MOLECULAR NUTRITION & FOOD RESEARCH | Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and?… [Pubmed: 32918393] |
Neuropharmacology | Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084] |
COLLOIDS AND SURFACES B-BIOINTERFACES | Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease [Pubmed: 32777662] |
博士論文 | Neurobiological determinants of depressive-like symptoms in rodents [] |
Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy [] |
Journal of the Taiwan Institute of Chemical Engineers | Multiple-component dual-phase solid lipid nanoparticles with conjugated transferrin for formulating antioxidants and nerve growth factor against neuronal apoptosis [] |
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free??- [33389158] |
DRUG DEVELOPMENT RESEARCH | Neuroprotective effect of Na+/H+?exchangers isoform?\1 inactivation against 6?\hydroxydopamine?\induced mitochondrial dysfunction and neuronal apoptosis in Parkinson's disease models [33538000] |
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways [33589896] |
Neuropsychiatr Dis Treat | Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567?… [34239303] |
Pharmaceuticals (Basel) | Carissa macrocarpa Leaves Polar Fraction Ameliorates Doxorubicin-Induced Neurotoxicity in Rats via Downregulating the Oxidative Stress and Inflammatory Markers [34959705] |
Cell Biochem Funct | CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway [34296452] |
Carbohydrate Polymers | Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration [Pubmed:35550777] |
留言咨詢