脂質運載蛋白2(NGAL)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Neutrophil gelatinase-associated lipocalin (NGAL)
Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
- 編號KSB388Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.055ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 4838 ¥ 8064 ¥ 14515 ¥ 28224 ¥ 48384
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特異性
試劑盒中的抗體對中性粒細胞明膠酶關聯脂質運載蛋白(NGAL)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
APB388Mu01 | 脂質運載蛋白2(NGAL)活性蛋白 | Cell?culture;?Activity?Assays. |
APB388Mu61 | 脂質運載蛋白2(NGAL)活性蛋白 | Cell?culture;?Activity?Assays. |
RPB388Mu02 | 脂質運載蛋白2(NGAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPB388Mu61 | 脂質運載蛋白2(NGAL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB388Mu01 | 脂質運載蛋白2(NGAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB388Mu01 | 脂質運載蛋白2(NGAL)多克隆抗體 | WB; IHC; ICC; IP. |
PAB388Mu02 | 脂質運載蛋白2(NGAL)多克隆抗體 | WB; IHC; ICC; IP. |
LAB388Mu81 | 脂質運載蛋白2(NGAL)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAB388Mu71 | 脂質運載蛋白2(NGAL)多克隆抗體(生物素標記) | WB; IHC; ICC. |
SEB388Mu | 脂質運載蛋白2(NGAL)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB388Mu | 脂質運載蛋白2(NGAL)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB388Mu | 脂質運載蛋白2(NGAL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB388Mu01 | 脂質運載蛋白2(NGAL)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
International Journal of Nephrology and Renovascular Disease | Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancy [PubMed: PMC3794962] |
International Journal of Endocrinology | Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 Pathway [Pubmed: 24369464] |
International Journal of Sport Studies | Lipocalin-2: Response To Resistance Training in Obese Young Men [Ijssjournal: Source] |
Asian Journal of Sports Medicine | Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young Men [Tums: Source] |
European Respiratory Journal | Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlap [Pubmed: 23794464] |
Acta Histochem.? | Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy [Pubmed:24802166] |
Int Urol Nephrol. | Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental study [Pubmed:25812822] |
世界華人消化雜志 | 血清及膽汁中NGAL 對于區(qū)分良惡性膽道狹窄的臨床意義 [Wjgnet:Source] |
Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats [Sagepub:Source] |
Biomedical & Pharmacology Journal | Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese Men [Biomedpharmajournal:Source] |
Asian Journal of Physical Education and Computer Science in Sports | A Study On Thyroid Hormone And Its Effects On Exercise [Ifcss:Source] |
臨床兒科雜志 | 中性粒細胞明膠酶相關脂質運載蛋白、腎損傷分子1和白介素18 在診斷心肺分流術后急性腎損傷中的意義 [Jcp-Sh:Source] |
Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancy [Pubmed:Pmc4260273] | |
Int J Chron Obstruct Pulmon Dis. | Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease [Pubmed:Pmc4043430] |
Pediatric Anesthesia? | Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model [Pubmed:24916275] |
Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
Int J Clin Exp Pathol | Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis [PubMed: 26191125] |
BMC Anesthesiol | Increased cardiac index attenuates septic acute kidney injury: a prospective observational study [PubMed: 25745357] |
中華臨床醫(yī)生雜志 | 中性粒細胞明膠酶相關脂質運載蛋白對腹膜透析相關性腹膜炎療效監(jiān)測的意義 [Journal: Fulltext] |
PLoS One | Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats [PubMed: 25909514] |
Pediatric Anesthesia | Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model [Pubmed:26714833] |
Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748] |
Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu] |
International Journal of Molecular Medicine | The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats [pubmed:27430552] |
Hepatology. | Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis. [pubmed:27775820] |
Lipids in Health and Disease | Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN) [pubmed:28659153] |
International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748] |
Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. [pubmed:29027259] |
Scientific Reports | Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery [Pubmed:29386545] |
Life?Sciences | Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity [Pubmed:30036488] |
Molecules | Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy [Pubmed:29642511] |
American Journal of Translational?Research | Improved approach for normothermic machine perfusion of cold stored kidney grafts [Pubmed:30018731] |
Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes [ISBN:978-951-51-4039-5] | |
Disease Markers | Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis [Doi: 10.1155/2018/1015726] |
Kidney?and?Blood?Pressure?Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713] |
BMC?Complementary and Alternative Medicine | Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine [Pubmed: 30871515] |
Naunyn-Schmiedeberg's archives of pharmacology | Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascade [Pubmed: 31197431] |
Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation [Pubmed: 31447572] |
Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605] |
Scientific Reports | Alkaline phosphatase treatment of Acute Kidney injury in an infant piglet Model of cardiopulmonary Bypass with Deep Hypothermic circulatory Arrest [Pubmed: 31578351] |
Int Urol Nephrol | The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial?… [Pubmed: 32980930] |
human & experimental toxicology | Upregulated miR-101 inhibits acute kidney injury–chronic kidney disease transition by regulating epithelial–mesenchymal transition [Pubmed: 32633566] |
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY | CОСТОЯНИЕ ТИРЕОИДНОЙ ФУНКЦИИ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ 1-ГО ТИПА И ХРОНИЧЕСКОЙ БОЛЕЗНЬЮ ПОЧЕК?… [] |
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY | Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy [] |
Pediatr Int | Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers [33423326] |
ARCHIVES OF TOXICOLOGY | Nrf2 deficiency aggravates the kidney injury induced by subacute cadmium exposure in mice [33398418] |
Biomed Res Int | Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury [33604384] |
International Journal of Medical Sciences | Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer [34104101] |
Intensive Care Medicine Experimental | Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental?… [34169407] |
Biomolecules | Preservation of Mitochondrial Coupling and Renal Function by Controlled Oxygenated Rewarming of Porcine Kidney Grafts [34944524] |
RSC Adv | SERS based Y-shaped aptasensor for early diagnosis of acute kidney injury [Pubmed:35733690] |
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