色素上皮衍生因子(PEDF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Pigment Epithelium Derived Factor (PEDF)
SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3
- 編號(hào)KSB972Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.39-25ng/mL
- 可適靈敏度0.18ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特異性
試劑盒中的抗體對(duì)色素上皮衍生因子(PEDF)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB972Mu01 | 色素上皮衍生因子(PEDF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB972Mu02 | 色素上皮衍生因子(PEDF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB972Mu03 | 色素上皮衍生因子(PEDF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB972Mu01 | 色素上皮衍生因子(PEDF)多克隆抗體 | WB; IHC; ICC; IP. |
PAB972Mu03 | 色素上皮衍生因子(PEDF)多克隆抗體 | WB; IHC; ICC; IP. |
PAB972Mu02 | 色素上皮衍生因子(PEDF)多克隆抗體 | WB; IHC; ICC; IP. |
LAB972Mu81 | 色素上皮衍生因子(PEDF)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAB972Mu71 | 色素上皮衍生因子(PEDF)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAB972Mu21 | 色素上皮衍生因子(PEDF)單克隆抗體 | WB,IHC |
MAB972Mu22 | 色素上皮衍生因子(PEDF)單克隆抗體 | WB; IHC; ICC; IP. |
MEB972Mu | 色素上皮衍生因子(PEDF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB972Mu | 色素上皮衍生因子(PEDF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB972Mu | 色素上皮衍生因子(PEDF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB972Mu | 色素上皮衍生因子(PEDF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB972Mu01 | 色素上皮衍生因子(PEDF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Molecular Therapy | A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions [Nature: 2010144] |
American Journal of Nephrology | Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy [Karger: 314326] |
34 | Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury [OxfordJournals: source] |
Heart and Education in Heart | The change of serum level of pigment epithelium-derived factor in coronary heart disease [Bmj: Source] |
Mediators of Inflammation | Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation [Pubmed: 24288442] |
Cardiology. | The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. [Pubmed: 24192856] |
Endocrinology | Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism [Endocrine: Source] |
Clinical Science | Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis [PubMed: 25700221] |
Molecular Sciences | Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R [Pubmed:27187377] |
Investigative ophthalmoloey & visual science | The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells [articleid:2582822] |
Scientific reports | VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice [pubmed:28091556] |
國(guó)際眼科雜志 | 色素上皮衍生因子修飾的人臍帶間充質(zhì)干細(xì)胞構(gòu)建方法 [201707007] |
Investigative?Ophthalmology?&?Visual?Science | PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice [Pubmed:30025136] |
International Eye Science | 高海拔地區(qū)新生血管性青光眼不同治療方式的療效及對(duì)房水中PEDF, VEGF 水平的影響 [2018/2/201802020.pdf] |
Domestic?Animal?Endocrinology | Lactation-related changes in tissue expression of PEDF in dairy cows [Pubmed:29758402] |
Medical?Science?Monitor | Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a?… [Pubmed:29574467] |
Chemosphere | Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis [Doi: 10.1016/j.chemosphere.2018.10.100] |
Biological?Trace?Element?Research | Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model [Pubmed: 30392020] |
International?Urology?and Nephrology | Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement [Pubmed: 30536192] |
International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807] |
Journal of the American Heart Association | Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia [Pubmed: 31711388] |
environmental research | Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells [Pubmed: 32315827] |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells [33498962] |
Hum Cell | Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ?- [33768511] |
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