S100鈣結(jié)合蛋白A12(S100A12)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for S100 Calcium Binding Protein A12 (S100A12)
CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6
- 編號KSB080Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍31.2-2,000pg/mL
- 可適靈敏度13.5pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對S100鈣結(jié)合蛋白A12(S100A12)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB080Hu01 | S100鈣結(jié)合蛋白A12(S100A12)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB080Hu01 | S100鈣結(jié)合蛋白A12(S100A12)多克隆抗體 | WB; IHC; ICC; IP. |
LAB080Hu81 | S100鈣結(jié)合蛋白A12(S100A12)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAB080Hu71 | S100鈣結(jié)合蛋白A12(S100A12)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAB080Hu21 | S100鈣結(jié)合蛋白A12(S100A12)單克隆抗體 | WB |
MAB080Hu23 | S100鈣結(jié)合蛋白A12(S100A12)單克隆抗體 | WB; IHC; ICC; IP. |
MAB080Hu24 | S100鈣結(jié)合蛋白A12(S100A12)單克隆抗體 | WB. |
MAB080Hu29 | S100鈣結(jié)合蛋白A12(S100A12)單克隆抗體 | WB; IHC; ICC; IP. |
MAB080Hu27 | S100鈣結(jié)合蛋白A12(S100A12)單克隆抗體 | WB; IHC; ICC; IP. |
MAB080Hu25 | S100鈣結(jié)合蛋白A12(S100A12)單克隆抗體 | WB,ICC/IF |
MAB080Hu26 | S100鈣結(jié)合蛋白A12(S100A12)單克隆抗體 | WB; IHC; ICC; IP. |
MAB080Hu22 | S100鈣結(jié)合蛋白A12(S100A12)單克隆抗體 | WB; IHC; ICC; IP. |
MEB080Hu | S100鈣結(jié)合蛋白A12(S100A12)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB080Hu | S100鈣結(jié)合蛋白A12(S100A12)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB080Hu | S100鈣結(jié)合蛋白A12(S100A12)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB080Hu01 | S100鈣結(jié)合蛋白A12(S100A12)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Inflammation Research | Correlation of human S100A12 (EN-RAGE) and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes [Pubmed: 24378957] |
Dig Dis Sci | The Circulating Level of Soluble Receptor for Advanced Glycation End Products Displays Different Patterns in Ulcerative Colitis and Crohn’s Disease: A Cross-Sectional Study [PubMed: 25757448] |
The Journal of Clinical Endocrinology & Metabolism | Low Endogenous Secretory Receptor for Advanced Glycation End-Products Levels Are Associated With Inflammation and Carotid Atherosclerosis in Prediabetes [Pubmed:26885882] |
Nutrition, Metabolism, and Cardiovascular Diseases | High intake of dietary advanced glycation end-products is associated with increased arterial stiffness and inflammation in subjects with type 2 diabetes. [pubmed:28958695] |
The Journal of Clinical Endocrinology & Metabolism | HbA1c Identifies Subjects With Prediabetes and Subclinical Left Ventricular Diastolic Dysfunction. [pubmed:28973588] |
Journal of Immunology Research | A Granulocyte-Specific Protein S100A12 as a Potential Prognostic Factor Affecting Aggressiveness of Therapy in Patients with Juvenile Idiopathic Arthritis [Doi: 10.1155/2018/5349837] |
Al-Azhar Assiut Medical Journal | The role of serum S100A12 (calgranulin C) as a diagnostic marker in Egyptian patients with irritable bowel syndrome and ulcerative colitis [Doi: 10.4103/AZMJ.AZMJ_52_18] |
Acta?Diabetologica | Analysis of S100A12 plasma levels in hyperlipidemic subjects with or without familial hypercholesterolemia [Pubmed: 30963307] |
Cells | 1 h Postload Glycemia Is Associated with Low Endogenous Secretory Receptor for Advanced Glycation End Product Levels and Early Markers of Cardiovascular?… [] |
Biomolecules . | Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study [Pubmed: 32549327] |
Mediators of Inflammation | Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris [] |
Animals (Basel) | The Serum and Saliva Proteome of Dogs with Diabetes Mellitus [33271797] |
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