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血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)

ADMP-2; Aggrecanase-2

  • 血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPK205Hu01 血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAK205Hu01 血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)多克隆抗體 WB; IHC; ICC; IP.
MAK205Hu21 血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)單克隆抗體 WB; IHC; ICC; IP.
SEK205Hu 血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMK205Hu 血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSK205Hu01 血小板反應(yīng)蛋白解整合素金屬肽酶5(ADAMTS5)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Osteoarthritis and Cartilage Lack of anti-inflammatory and anti-catabolic effects on basal inflamed osteoarthritic chondrocytes or synoviocytes by adipose stem cell-conditioned medium [PubMed: 26521751]
Journal of Cellular and Molecular Medicine VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts [Pubmed:26818776]
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748]
The American journal of pathology Healthy and Osteoarthritic Synovial Fibroblasts Produce a Disintegrin and Metalloproteinase with Thrombospondin Motifs 4, 5, 7, and 12: Induction by IL-1β and Fibronectin and Contribution to Cartilage Damage. [pubmed:27449198]
PLoS One. Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. [pubmed:28207788]
PLoS One Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders [10.1371:journal.pone.0172181]
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748]
BMC musculoskeletal disorders Hemoglobin stimulates the expression of ADAMTS-5 and ADAMTS-9 by synovial cells: a possible cause of articular cartilage damage after intra-articular hemorrhage. [pubmed:29137610]
Journal of Clinical Medicine The Effects of TNF-α Inhibition on the Metabolism of Cartilage: Relationship between KS, HA, HAPLN1 and ADAMTS4, ADAMTS5, TOS and TGF-β1 Plasma?… [Pubmed:35407621]
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