α2-HS糖蛋白(AHSG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Alpha-2-Heremans Schmid Glycoprotein (AHSG)
aHSG; HSGa; A2HS; AHS; FETUA; HSG-A2; Fetuin-A; Alpha 2-HS Glycoprotein; Fetuin A; Alpha-2-Z-Globulin; Ba-alpha-2-Glycoprotein
- 編號KSA178Bo01
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍15.6-1000ng/mL
- 可適靈敏度7.1ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 8165 ¥ 13608 ¥ 24494 ¥ 47628 ¥ 81648
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對α2-HS糖蛋白(aHSG)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA178Bo01 | α2-HS糖蛋白(AHSG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA178Bo01 | α2-HS糖蛋白(AHSG)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA178Bo21 | α2-HS糖蛋白(AHSG)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA178Bo | α2-HS糖蛋白(AHSG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA178Bo | α2-HS糖蛋白(AHSG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA178Bo01 | α2-HS糖蛋白(AHSG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
| PGA178Bo01 | α2-HS糖蛋白(AHSG)引物對 | PCR |
| CGA178Bo01 | α2-HS糖蛋白(AHSG)cDNA | PCR、蛋白表達等 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Proteomics | Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systems [ScienceDirect: S1874391912002084] |
| Journal of Physiology and Biochemistry | Curcumin prevents liver fat accumulation and serum fetuin-A increase in rats fed a high-fat diet [PubMed: 23430567] |
| BioMed Research International | Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats [PubMed: PMC3591142] |
| Osteoporosis International | Estrogen increases the transcription of human α2-Heremans-Schmid-glycoprotein by an interplay of estrogen receptor α and activator protein-1. [Pubmed: 24504099] |
| BMC Pharmacology and Toxicology | A novel 11β-hydroxysteroid dehydrogenase type1 inhibitor CNX-010-49 improves hyperglycemia, lipid profile and reduces body weight in diet induced obese C57B6/J mice with a potential to provide cardio protective benefits [Pubmed:25098735] |
| Acta Dermatovenerologica Croatica | Relationship Between Serum Fetuin-A Levels and Carotid Intima-media Thickness in Turkish Patients with Mild to Moderate Psoriasis. A Case-control Study [PubMed: 26476900] |
| Mol Cell Endocrinol | Fetuin A promotes lipotoxicity in β cells through the TLR4 signaling pathway and the role of pioglitazone in anti-lipotoxicity. [PubMed: 25986658] |
| Ann Dermatol | Evaluation of Arterial Stiffness in Patients with Beh [PubMed: 26719639] |
| Endocrine | Visceral and subcutaneous adipose tissue express and secrete functional alpha2hsglycoprotein (fetuin a) especially in obesity [pubmed:27738888] |
| Diabetes Care | Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans [pubmed:29212824] |
| JOURNAL OF?ALZHEIMERS?DISEASE | Acute Anti-Inflammatory Markers ITIH4 and AHSG in Mice Brain of a Novel Alzheimer's Disease Model [Pubmed: 30958362] |
| Journal of Alzheimer's Disease | A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology [Pubmed: 31771070] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| J Obstet Gynaecol Res | Pred?ct?ve value of pregnancy of foll?cular flu?d fetu?n‐A and‐B levels ?n infert?le women after intra‐cytoplasmic sperm injection [34708901] |
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