膜聯(lián)蛋白A1(ANXA1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Annexin A1 (ANXA1)
ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein
- 編號(hào)KSE787Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍6.25-400ng/mL
- 可適靈敏度2.87ng/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 8208 ¥ 13680 ¥ 24624 ¥ 47880 ¥ 82080
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)膜聯(lián)蛋白A1(ANXA1)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPE787Ra02 | 膜聯(lián)蛋白A1(ANXA1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPE787Ra01 | 膜聯(lián)蛋白A1(ANXA1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APE787Ra01 | 膜聯(lián)蛋白A1(ANXA1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAE787Ra01 | 膜聯(lián)蛋白A1(ANXA1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAE787Ra71 | 膜聯(lián)蛋白A1(ANXA1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAE787Ra27 | 膜聯(lián)蛋白A1(ANXA1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE787Ra21 | 膜聯(lián)蛋白A1(ANXA1)單克隆抗體 | WB,IHC |
| MAE787Ra22 | 膜聯(lián)蛋白A1(ANXA1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE787Ra23 | 膜聯(lián)蛋白A1(ANXA1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE787Ra24 | 膜聯(lián)蛋白A1(ANXA1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE787Ra25 | 膜聯(lián)蛋白A1(ANXA1)單克隆抗體 | WB; IHC; ICC; IP. |
| FAE787Ra04 | 抗膜聯(lián)蛋白A1(ANXA1)單克隆抗體 | Flow cytometry. |
| FAE787Ra02 | 抗膜聯(lián)蛋白A1(ANXA1)單克隆抗體 | Flow cytometry. |
| SEE787Ra | 膜聯(lián)蛋白A1(ANXA1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME787Ra | 膜聯(lián)蛋白A1(ANXA1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSE787Ra01 | 膜聯(lián)蛋白A1(ANXA1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Plos one | Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease [PubMed: PMC3794972] |
| African Journals Online | Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2 [Ajol: Source] |
| Antimicrobial Agents and Chemotherapy | Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic Response [Asm: Source] |
| J Proteomics | Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) [PubMed: 26718731] |
| Diabetes | Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet Function [PubMed: 26470781] |
| PLoS One | Annexin A1 Is Increased in the Plasma of Preeclamptic Women [PubMed: 26398190] |
| 中國(guó)現(xiàn)代醫(yī)學(xué)雜志 China Journal of Modern Medicine | 膜聯(lián)蛋白A1 和CA19-9 在膽管癌中的表達(dá)及其臨床意義 [article:8686] |
| arteriosclerosis thrombosis and vascular biology | Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. [pubmed:28062503] |
| Clinical Science | Mesenchymal stromal cells improve human islet function through released products and extracellular matrix. [pubmed:29101297] |
| Biomedicine & Pharmacotherapy | Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity [Pubmed:29677533] |
| ?International Journal of?Chronic?Obstructive Pulmonary Disease | Annexin A1 is elevated in patients with COPD and affects lung fibroblast function [Pubmed:29440885] |
| Medical?Mycology | Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients [Pubmed:29370415] |
| DEVELOPMENTAL NEUROPSYCHOLOGY | EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT?… [pdf:3395] |
| Multiple sclerosis and related disorders | Resolution of inflammation in neuromyelitis optica spectrum disorders [Pubmed: 30300851] |
| Acta Pharmaceutica | Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines [] |
| npj Science of Food | Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites [Pubmed: 32133417] |
| FASEB JOURNAL | ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway [Pubmed: 32908567] |
| Rev Soc Bras Med Trop | Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients [33263684] |
| science advances | Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK|á stability via selective autophagy [33523920] |
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