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天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Aspartate Aminotransferase (AST)

cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble

  • 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對天冬氨酸轉(zhuǎn)氨酶(AST)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB214Ra01 天冬氨酸轉(zhuǎn)氨酶(AST)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB214Ra01 天冬氨酸轉(zhuǎn)氨酶(AST)多克隆抗體 WB; IHC; ICC; IP.
LAB214Ra71 天冬氨酸轉(zhuǎn)氨酶(AST)多克隆抗體(生物素標記) WB; IHC; ICC.
MAB214Ra22 天冬氨酸轉(zhuǎn)氨酶(AST)單克隆抗體 WB; IHC; ICC; IP.
MAB214Ra21 天冬氨酸轉(zhuǎn)氨酶(AST)單克隆抗體 WB; IHC; ICC; IP.
SEB214Ra 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB214Ra 天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB214Ra01 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms [Pubmed: 23325074]
European Journal of Pharmacology Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats [ScienceDirect: S0014299914000235]
Journal of Immunology The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice [Pubmed:25480563]
Gastroenterol Res Pract Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning [PubMed: 26697061]
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?Biochimica et Biophysica Acta (BBA)-General Subjects Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485]
Scientific Reports Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency [Pubmed:29379059]
FEBS?Open?Bio A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis [Pubmed:29321963]
BMC?Complementary and Alternative?Medicine? Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910]
laboratory investigation Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiency [Pubmed: 31186526]
Brain Behavior and Immunity Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy [Pubmed: 31437533]
Biomed Research International Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099]
Nanomaterials Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313]
European Journal of Biology Effects of Tempol in Lipopolysaccharide-Induced Liver Injury []
Pathogens Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus [33925018]
Int Immunopharmacol Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes [34848154]
Acta Pharmacol Sin NFAT inhibitor 11R-VIVIT ameliorates mouse renal fibrosis after ischemia-reperfusion-induced acute kidney injury [34937917]
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Medcomm Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and?… [Pubmed:35548710]
International Immunopharmacology Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation [Pubmed:35605433]
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