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上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78)

CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78

  • 上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)上皮中性粒細(xì)胞激活肽78(ENA78)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA860Mu02 上皮中性粒細(xì)胞激活肽78(ENA78)活性蛋白 Cell?culture;?Activity?Assays.
RPA860Mu02 上皮中性粒細(xì)胞激活肽78(ENA78)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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RPA860Mu01 上皮中性粒細(xì)胞激活肽78(ENA78)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA860Mu02 上皮中性粒細(xì)胞激活肽78(ENA78)多克隆抗體 WB; IHC; ICC; IP.
PAA860Mu01 上皮中性粒細(xì)胞激活肽78(ENA78)多克隆抗體 WB; IHC; ICC; IP.
LAA860Mu81 上皮中性粒細(xì)胞激活肽78(ENA78)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA860Mu71 上皮中性粒細(xì)胞激活肽78(ENA78)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
SEA860Mu 上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA860Mu 抗上皮中性粒細(xì)胞激活肽78抗體(Anti-ENA78)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA860Mu 上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA860Mu 上皮中性粒細(xì)胞激活肽78(ENA78)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA860Mu01 上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Biochemical and Molecular Toxicology Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis. [Pubmed:26862043]
Journal of Biochemical and Molecular Toxicology Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis [pubmed:26862043]
OncoTargets and Therapy The clinical significance of CXCL5 in non-small cell lung cancer. [pubmed:29200871]
Biochimie Activated CXCL5-CXCR2 axis promotes the migration, invasion and EMT of papillary thyroid carcinoma cells via modulation of β-catenin pathway [Pubmed:29471001]
Cancer?Biology?&?Therapy Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38?… [Pubmed: 30404567]
Respiratory?Research Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from?… [Pubmed: 30634975]
American Journal of Pathology Interleukin17–CXCR2 axis facilitates breast cancer progression by up-regulating neutrophil recruitment [Pubmed: 31654638]
Bioactive Materials Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy [34820587]
Am J Cancer Res Differential expression profile of CXC-receptor-2 ligands as potential biomarkers in pancreatic ductal adenocarcinoma [Pubmed:35141005]
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