脂肪酸合酶(FASN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Fatty Acid Synthase (FASN)
FAS; OA519; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X,Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase
- 編號KSC470Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.116ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對脂肪酸合酶(FASN)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPC470Hu02 | 脂肪酸合酶(FASN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC470Hu01 | 脂肪酸合酶(FASN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC470Hu02 | 脂肪酸合酶(FASN)多克隆抗體 | WB; IHC; ICC; IP. |
PAC470Hu01 | 脂肪酸合酶(FASN)多克隆抗體 | WB; IHC; ICC; IP. |
MAC470Hu22 | 脂肪酸合酶(FASN)單克隆抗體 | WB; IHC; ICC; IP. |
SEC470Hu | 脂肪酸合酶(FASN)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC470Hu | 脂肪酸合酶(FASN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSC470Hu01 | 脂肪酸合酶(FASN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
Oncogenesis. | Diet-induced alteration of fatty acid synthase in prostate cancer progression [Pubmed:26878389] |
Animal Science Journal | Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells [pubmed:28804941] |
life?sciences | Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma [Pubmed: 30914315] |
Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights?… [] |
bmc research notes | The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers [Pubmed: 32698888] |
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