甘丙肽(GAL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Galanin (GAL)
GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide
- 編號KSB084Ra11
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度4.97pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對甘丙肽(GAL)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPB084Ra01 | 甘丙肽(GAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB084Ra01 | 甘丙肽(GAL)多克隆抗體 | WB; IHC; ICC; IP. |
MAB084Ra21 | 甘丙肽(GAL)單克隆抗體 | WB; IHC; ICC; IP. |
CEB084Ra | 甘丙肽(GAL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCB084Ra | 甘丙肽(GAL)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB084Ra | 甘丙肽(GAL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB084Ra11 | 甘丙肽(GAL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Progress in Neuro-Psychopharmacology and Biological Psychiatry | Decreased galanin serum levels are associated with alcohol-craving during withdrawal [ScienceDirect: S0278584610005191] |
Clinical Biochemistry | Circulating galanin levels are increased in patients with gestational diabetes mellitus [PubMed: 23266294] |
Peptides | Endogenous galanin as a novel biomarker to predict gestational diabetes mellitus. [Pubmed: 24503374] |
Gynecological Endocrinology | Circulating galanin and IL-6 concentrations in gestational diabetes mellitus [Pubmed:24397394] |
Eat Behav | Association between circulating levels of galanin and pre-pregnancy body mass index in patients with gestational diabetes mellitus [PubMed: 26172564] |
Cell Biochemistry and Biophysics | The Effects of Levetiracetam on Cerebrospinal Fluid and Plasma NPY and GAL, and on the Components of Stress Response System, hs-CRP, and S100B Protein in Serum of Patients with Refractory Epilepsy [Article: 10.1007] |
Journal of Addiction & Prevention | Low Galanin Serum Levels are Associated with Anxiety in Opioid-Maintained Patients [688687ca8d5a1d03ed060f761328001ca673.pdf] |
Oncology Letter | Colorectal cancer patients exhibit increased levels of galanin in serum and colon tissues [pubmed:27899999] |
Scientific Reports | Serum?Galanin?Levels?in?Young?Healthy?Lean?and?Obese?Non-Diabetic?Men?during an?OralGlucose?Tolerance?Test. [pubmed:27550417] |
Journal of?Clinical?Investigation? | Adrenomedullin and Galanin Responses to Orthostasis in Older Persons [pubmed:28796366] |
Child Psychiatry Hum Dev | Serum Galanin in Children with Autism Spectrum Disorder [33534034] |
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