葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Glucose Transporter 4 (GLUT4)
SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive
- 編號(hào)KSC023Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Tissue homogenates, cell lysates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍1.25-20ng/mL
- 可適靈敏度0.51ng/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC023Hu01 | 葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC023Hu01 | 葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)多克隆抗體 | WB; IHC; ICC; IP. |
| MAC023Hu22 | 葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC023Hu | 葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC023Hu | 葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC023Hu01 | 葡萄糖轉(zhuǎn)運(yùn)蛋白4(GLUT4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Polar Record | Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker development [Cambridge: Source] |
| The International Journal of Biochemistry & Cell Biology | Conditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogs [Pubmed:25236492] |
| Psychoneuroendocrinology | Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats [PubMed: 26143539] |
| PLOS ONE | Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug [PubMed: 26262991] |
| American Journal of Animal and Veterinary Sciences | Metabolic Features of Heart Failure with Different Etiology [ofsp11075] |
| Journal of Pharmaceutical Negative Results | Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation [Doi: 10.4103/jpnr.JPNR_14_18] |
| eureka herba indonesia | Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue [] |
| eureka herba indonesia | The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study [] |
| eureka herba indonesia | Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan [] |
| Eureka Herba Indonesia | Potential Combination of Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia Extracts Against??- [] |
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