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硫酸肝素蛋白聚糖(HSPG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Heparan Sulfate Proteoglycan (HSPG)

SDC2; HSPG1; SYND2; Fibroglycan; Syndecan 2; Syndecan Proteoglycan 2; Heparan Sulfate Proteoglycan 1,Cell Surface-Associated; Heparan Sulphate Proteoglycan

  • 硫酸肝素蛋白聚糖(HSPG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 硫酸肝素蛋白聚糖(HSPG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)硫酸肝素蛋白聚糖(HSPG)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA565Hu01 硫酸肝素蛋白聚糖(HSPG)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA565Hu01 硫酸肝素蛋白聚糖(HSPG)多克隆抗體 WB; IHC; ICC; IP.
LAA565Hu71 硫酸肝素蛋白聚糖(HSPG)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
LAA565Hu81 硫酸肝素蛋白聚糖(HSPG)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
MAA565Hu22 硫酸肝素蛋白聚糖(HSPG)單克隆抗體 WB; IHC; ICC; IP.
SEA565Hu 硫酸肝素蛋白聚糖(HSPG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA565Hu 硫酸肝素蛋白聚糖(HSPG)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA565Hu01 硫酸肝素蛋白聚糖(HSPG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Applied Pharmaceutical Science Cytotoxic effects of antiglypican-3 against HepG3 cell line [Ebscohost: Source]
Journal of Reproductive Immunology Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patients [Pubmed: 24661950]
European journal of pharmacology Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme [Pubmed: 24530413]
European Journal of Pharmacology Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma [Pubmed:25449037]
Shock Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257]
Biomarkers in Medicine Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669]
Apmis Circulating syndecans during critical illness. [pubmed:28256016]
Biomedicine & Pharmacotherapy Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and [Pubmed:29669302]
Drug?Delivery Glioma targeting peptide modified apoferritin nanocage [Pubmed:29726297]
Anaesthesia Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass [Pubmed: 30687934]
Animals Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period … []
LIFE SCIENCES Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients [33781826]
Genet Mol Biol A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate [34042151]
Poultry Science Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine??- [33518110]
J Gerontol A Biol Sci Med Sci The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo?… [34423832]
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